Nitrocellulose Membranes, 0.45 μm
Nitrocellulose Membranes, 0.45 μm
Thermo Scientific™

Nitrocellulose Membranes, 0.45 μm

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）Dimensions (LxW)フォーマット対応可能
7701012 cm x 8 cmシート25 Mini-gel Blots
880258 cm x 8 cmシート15 ミニゲルブロッティング
8801410.5 cm x 7.9 cmシート15 Mini-gel Blots
8801830 cm x 3.5 mRoll8 × 10 cm にカットする場合は、少なくとも 120 ミニゲルブロッティング
製品番号（カタログ番号） 77010
価格（JPY）
81,100
Each
お問い合わせください ›
Dimensions (LxW):
12 cm x 8 cm
フォーマット:
シート
対応可能:
25 Mini-gel Blots
一括またはカスタム形式をリクエストする
Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.45-μm pore size is well-suited for a wide range of western blotting conditions and ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

Features include:
• 100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets or choose the roll format for the flexibility to cut to the ideal size
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background: produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

For Research Use Only. Not for use in diagnostic procedures.
仕様
結合能∼80 μg/cm2 of protein
概要Pierce ニトロセルロースメンブレン、0.45 um、8 cm × 12 cm
数量25 Pre-cut Membranes
出荷条件Room Temperature
Dimensions (LxW)12 cm x 8 cm
フォーマットシート
長さ（メートル法）12 cm
材料ニトロセルロース膜
孔径0.45 μm
対応可能25 Mini-gel Blots
幅（メートル法）8 cm
Unit SizeEach
組成および保存条件
Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle. Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.

よくあるご質問（FAQ）

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.