Imject™ cBSA (in PBS)
Imject™ cBSA (in PBS)
Thermo Scientific™

Imject™ cBSA (in PBS)

Thermo Scientific Imjectカチオン化ウシ血清アルブミン(cBSA)は、抗体産生用の抗原調製に用いられる、使いやすく免疫原性の高いキャリアタンパク質です。Imject cBSAでは、ネイティブなBSAが大過剰のエチレンジアミンで修飾され詳細を見る
製品番号(カタログ番号)数量
7715010 mg
製品番号(カタログ番号) 77150
価格(JPY)
56,000
Online offer
Ends: 26-Dec-2025
93,400
割引額 37,400 (40%)
Each
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数量:
10 mg
Thermo Scientific Imjectカチオン化ウシ血清アルブミン(cBSA)は、抗体産生用の抗原調製に用いられる、使いやすく免疫原性の高いキャリアタンパク質です。

Imject cBSAでは、ネイティブなBSAが大過剰のエチレンジアミンで修飾され、負電荷のカルボキシル基が正電荷の一級アミンに変換されています。その結果、ネイティブ BSA よりも免疫原性の著しく向上した、高正荷電のタンパク質 (pI >11) が得られます。また、第一級アミンの数が増加すると、一般的な架橋法でさらに多数の抗原分子を結合させることができます。BSAより強力な免疫原性が必要だが、キーホールリンペットヘモシアニン(KLH)またはBlueキャリアタンパク質を使用できない場合、免疫原の調製にImject cBSAが有用です。

Imject Cationized Bovine Serum Albuminの特長:

•PBS中でカチオン化され、凍結乾燥した精製ウシ血清アルブミン(BSA)
• 分子量が67,000ダルトンを超える単純タンパク質
• 59個のリジン残基、およびカチオン化によって加えられた多数の一級アミンのほとんどが、抗原を付加する結合試薬に利用可能
• 純正電荷(pI 11超)により、負電荷を持つネイティブBSA(pI 5.1)に適さない条件で高い溶解性を示す
• 正電荷のタンパク質が免疫応答によって別に処理されるため、ネイティブBSAよりも高い免疫原性(参考文献を参照)。

キャリアタンパク質は大きく複雑な分子で、注入することで免疫応答を誘起できます。小さな抗原(ペプチドまたは薬物化合物)に対する特異的な抗体を確実に生成するには、これらハプテンをより大きく複雑な分子(通常はタンパク質)に共有結合させ、免疫原性を高める必要があります。キャリアタンパク質は免疫原性、可溶性、およびキャリアとの十分なコンジュゲーションが達成されるかによって選択されます。Imject Cationized BSA(cBSA)(以前の呼称:「SuperCarrier Protein」)は、水性バッファーに容易に溶解し、グルタルアルデヒド、EDC、その他の架橋試薬の標的となる多数の1級アミンを含んでいます。スルフヒドリル標的コンジュゲーション(例えばシステインを含むペプチド抗原)には、Imjectマレイミド活性化cBSAを使用します。

関連製品
Imject™ BSA (in PBS)
研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量10 mg
製品ラインImject
Unit SizeEach
組成および保存条件
4℃

よくあるご質問(FAQ)

What do I need to consider when designing a peptide for antibody production?

We recommend that hydrophobic residues comprise 50% or less of all the residues in your sequence. Make sure there is at least one charged residue for every five amino acids: this is generally known to enhance the solubility of the peptide. Peptides (compared to polypeptides, which fold and bury the hydrophobic amino acids) are too small to fold, so just a few hydrophobic amino acids may leave them insoluble.

Other guidelines include:
Peptides containing multiple Cys, Met, and Trp can be hard to synthesize.
Some sequences are problematic in solid-phase peptide synthesis or cleavage and are best avoided; these include Asp-Pro sequences or stretches of amino acids that require bulky protecting groups on their side chains during synthesis.
Glycine is often good for antigenicity as it has only a hydrogen side chain; this allows for complete rotation.
Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What is the optimal peptide length for antibody production?

Most peptide antigens range in length from 12 to 16 residues and are relatively easy to synthesize. Peptides of 9 residues or shorter have been effective antigens for antibody production, but peptides longer than 16 amino acids may contain several epitopes and form secondary structures. Peptides in excess of 18 residues begin to present more synthetic challenges. Before you synthesize your peptide, we recommend doing a BLASTP search using your peptide sequence. This is to make sure that the peptide is not homologous or identical to a sequence in a completely unrelated protein in the host animal.

Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What happens if antibodies are stored improperly?

Improper storage of antibodies can lead to:
- Degradation: Loss of activity and specificity
- Aggregation: Formation of precipitates or clumps
- Reduced performance: Poor results in assays or experiments

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do antibody pair kits contain, how many samples can I process, and how can I find a list of antibody pair kits by target?

Antibody pair kits contain capture antibody, detection antibody, recombinant standard and HRP conjugate. Each contains enough reagents to process forty 96-well plates. A list of Antibody Pair Kits (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) is available by target.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do Reagent Sets contain, how many samples can I process, and how can I check if a Reagent Set is available for my target?

Reagent Sets contain Capture Antibody, Detection Antibody, Recombinant Standard, HRP Conjugate, TMB Substrate and Stop Solution. Each contains enough reagents to process five 96-well plates. Reagent Sets are included in the main list of ELISA (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) kits (search by “Reagent Set”).

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.