Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Citations & References (5)

Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Name: Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
SKU: 78443

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製品番号（カタログ番号）	数量
78443	24 x 100 μL
78441	1 mL
78445	5 x 1 mL
78447	10 mL

4 オプション

製品番号（カタログ番号） 78443

価格（JPY）

68,900

Each

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数量:

24 x 100 μL

一括またはカスタム形式をリクエストする

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)は、1つの溶液で細胞および組織ライセートのタンパク質サンプルを完全に保護します。

Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)の特長：

•完全な保護—オールインワンカクテルはプロテアーゼとホスファターゼの両阻害剤を含有
• 複数のパッケージサイズ—4つの便利なパッケージサイズから選択できるため、ほぼすべての実験規模に適合
• 便利—100倍濃縮組成は、個別の阻害剤やその他のフォ＝マットよりも使いやすく効果的
• 保存が容易—水性フォーマットはDMSOベースのカクテルとは異なり冷凍する必要がなく、低温室で保存可能
• 適合性—ピークシフトを引き起こす可能性のあるAEBSFを含んでいないため、質量分析（MS）に使用可能
• 検証済み—Thermo Scientific Pierce Cell Lysis Bufferに完全に適合に完全に適合

Halt Protease and Phosphatase Inhibitor Cocktailは、タンパク質の抽出や精製中にタンパク質が分解されるのを防ぐよう、特別に最適化されています。カクテルには、アミノペプチダーゼ、システインおよびセリンプロテアーゼ、セリン／スレオニンおよびタンパク質チロシンホスファターゼを標的とする主要分類のプロテアーゼおよびホスファターゼに対する阻害剤が含まれます。

関連製品
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

研究用途にのみご使用ください。診断目的には使用できません。

仕様

バッファーLysis Buffer

概要Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free

阻害剤プロテアーゼ阻害剤、ホスファターゼ阻害剤

数量24 x 100 μL

試薬タイププロテアーゼ阻害剤、ホスファターゼ阻害剤

形状液体

製品ラインHalt

製品タイプProtease and Phosphatase Inhibitor Cocktail

Unit SizeEach

組成および保存条件

受け取り後、4℃で保存

よくあるご質問（FAQ）

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail, EDTA-Free (100X), 24 x 100 μL FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

Proteomic profiling across breast cancer cell lines and models.

Authors:Kalocsay M,Berberich MJ,Everley RA,Nariya MK,Chung M,Gaudio B,Victor C,Bradshaw GA,Eisert RJ,Hafner M,Sorger PK,Mills CE,Subramanian K

Journal:Scientific data

PubMed ID:37542042

We performed quantitative proteomics on 60 human-derived breast cancer cell line models to a depth of ~13,000 proteins. The resulting high-throughput datasets were assessed for quality and reproducibility. We used the datasets to identify and characterize the subtypes of breast cancer and showed that they conform to known transcriptional subtypes, ... More

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.

Authors:Nelson TJ,Xu Y

Journal:Scientific reports

PubMed ID:37221295

Alzheimer's disease (AD) is the most common cause of dementia. A common finding in AD is DNA damage. Double-strand DNA breaks (DSBs) are particularly hazardous to neurons because their post-mitotic state forces neurons to rely on error-prone and potentially mutagenic mechanisms to repair DNA breaks. However, it remains unclear whether ... More

FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.

Authors:Bartley CM,O'Keefe RA,Bordey A

Journal:PloS one

PubMed ID:24806451

Hyperactive mammalian target of rapamycin (mTOR) is associated with cognitive deficits in several neurological disorders including tuberous sclerosis complex (TSC). The phosphorylation of the mRNA-binding protein FMRP reportedly depends on mTOR complex 1 (mTORC1) activity via p70 S6 kinase 1 (S6K1). Because this phosphorylation is thought to regulate the translation ... More

Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.

Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P

Journal:Clin Chem

PubMed ID:23014600

The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More

The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.

Authors:Id Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F

Journal:BMC Cancer

PubMed ID:20565769

The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More