Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Citations & References (6)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

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製品番号（カタログ番号）数量
7844710 mL
784411 mL
7844324 x 100 μL
784455 x 1 mL
製品番号（カタログ番号） 78447
価格（JPY）
233,500
Each
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数量:
10 mL
一括またはカスタム形式をリクエストする
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)は、1つの溶液で細胞および組織ライセートのタンパク質サンプルを完全に保護します。

Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)の特長：

•完全な保護—オールインワンカクテルはプロテアーゼとホスファターゼの両阻害剤を含有
• 複数のパッケージサイズ—4つの便利なパッケージサイズから選択できるため、ほぼすべての実験規模に適合
• 便利—100倍濃縮組成は、個別の阻害剤やその他のフォ＝マットよりも使いやすく効果的
• 保存が容易—水性フォーマットはDMSOベースのカクテルとは異なり冷凍する必要がなく、低温室で保存可能
• 適合性—ピークシフトを引き起こす可能性のあるAEBSFを含んでいないため、質量分析（MS）に使用可能
• 検証済み—Thermo Scientific Pierce Cell Lysis Bufferに完全に適合に完全に適合

Halt Protease and Phosphatase Inhibitor Cocktailは、タンパク質の抽出や精製中にタンパク質が分解されるのを防ぐよう、特別に最適化されています。カクテルには、アミノペプチダーゼ、システインおよびセリンプロテアーゼ、セリン／スレオニンおよびタンパク質チロシンホスファターゼを標的とする主要分類のプロテアーゼおよびホスファターゼに対する阻害剤が含まれます。

関連製品
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
研究用途にのみご使用ください。診断目的には使用できません。
仕様
バッファーLysis Buffer
概要Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
阻害剤プロテアーゼ阻害剤、ホスファターゼ阻害剤
数量10 mL
試薬タイププロテアーゼ阻害剤、ホスファターゼ阻害剤
形状液体
製品ラインHalt
製品タイプProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
組成および保存条件
受け取り後4℃で保存。製品はアイスパック入りで出荷されます。製品を冷凍しないでください。

よくあるご質問（FAQ）

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 10 mL FAQs

引用および参考文献 (6)

引用および参考文献
Abstract
2-Aminopyridine-Based Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Inhibitors: Assessment of Mechanism-Based Safety.
Authors:Dow RL,Ammirati M,Bagley SW,Bhattacharya SK,Buckbinder L,Cortes C,El-Kattan AF,Ford K,Freeman GB,Guimarães CRW,Liu S,Niosi M,Skoura A,Tess D
Journal:Journal of medicinal chemistry
PubMed ID:29570292
Studies have linked the serine-threonine kinase MAP4K4 to the regulation of a number of biological processes and/or diseases, including diabetes, cancer, inflammation, and angiogenesis. With a majority of the members of our lead series (e.g., 1) suffering from time-dependent inhibition (TDI) of CYP3A4, we sought design avenues that would eliminate ... More
Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome.
Authors:Li Q,Zhang Q,Kim YR,Gaddam RR,Jacobs JS,Bachschmid MM,Younis T,Zhu Z,Zingman L,London B,Rauckhorst AJ,Taylor EB,Norris AW,Vikram A,Irani K
Journal:Nature communications
PubMed ID:37696839
Downregulation of endothelial Sirtuin1 (Sirt1) in insulin resistant states contributes to vascular dysfunction. Furthermore, Sirt1 deficiency in skeletal myocytes promotes insulin resistance. Here, we show that deletion of endothelial Sirt1, while impairing endothelial function, paradoxically improves skeletal muscle insulin sensitivity. Compared to wild-type mice, male mice lacking endothelial Sirt1 (E-Sirt1-KO) ... More
Mito-Mendelian interactions alter in vivo glucose metabolism and insulin sensitivity in healthy mice.
Authors:Sammy MJ,Connelly AW,Brown JA,Holleman C,Habegger KM,Ballinger SW
Journal:American journal of physiology. Endocrinology and metabolism
PubMed ID:34370595
The regulation of euglycemia is essential for human health with both chronic hypoglycemia and hyperglycemia having detrimental effects. It is well documented that the incidence of type 2 diabetes increases with age and exhibits racial disparity. Interestingly, mitochondrial DNA (mtDNA) damage also accumulates with age and its sequence varies with ... More
Deciphering the interactome of Ataxin-2 and TDP-43 in iPSC-derived neurons for potential ALS targets.
Authors:Tian Y,Heinsinger N,Hu Y,Lim UM,Wang Y,Fernandis AZ,Parmentier-Batteur S,Klein B,Uslaner JM,Smith SM
Journal:PloS one
PubMed ID:39739690
Ataxin-2 is a protein containing a polyQ extension and intermediate length of polyQ extensions increases the risk of Amyotrophic Lateral Sclerosis (ALS). Down-regulation of Ataxin-2 has been shown to mitigate TDP-43 proteinopathy in ALS models. To identify alternative therapeutic targets that can mitigate TDP-43 toxicity, we examined the interaction between ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
View all Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 10 mL citations