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Pierce™ IP Lysis Buffer
Pierce™ IP Lysis Buffer
Citations & References (3)
Thermo Scientific™

Pierce™ IP Lysis Buffer

Thermo Scientific Pierce IP Lysis Bufferは、細胞ライセートの高い収量と純度を確保し、細胞ライセートのダウンストリームアプリケーションとして免疫沈降（IPおよびCo-IP）に適合するよう最適化されています詳細を見る
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製品番号（カタログ番号）数量
87787100 mL
87788250 mL
製品番号（カタログ番号） 87787
価格（JPY）
28,200
Each
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数量:
100 mL
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Thermo Scientific Pierce IP Lysis Bufferは、細胞ライセートの高い収量と純度を確保し、細胞ライセートのダウンストリームアプリケーションとして免疫沈降（IPおよびCo-IP）に適合するよう最適化されています。

IP Lysis Bufferの特長：

•免疫沈降およびプルダウンアッセイに適合するように最適化済み
• タンパク質アッセイ、レポーターアッセイ、およびイムノアッセイ手順に適合
• 既製の製剤は、細胞質、膜、および核タンパク質の抽出に効果的
• 穏やかな製剤であるため、免疫共沈降（co-IP）のためにタンパク質複合体を維持
• サンプルの粘性を増加させる可能性のあるゲノムDNAを遊離させない

IP Lysis Bufferは、改変RIPAバッファー組成に基づいた、SDSを含有しない哺乳類全細胞溶解試薬です。この中強度の溶解バッファーは、細胞内タンパク質を効率的に可溶化しますが、通常のRIPAバッファーとは異なり、ゲノムDNAを遊離させたり、タンパク質複合体を破壊しません。Pierce IP Lysis Bufferは、プルダウンおよび免疫沈降アッセイ用に特別に調製されています。

Pierce IP Lysis Bufferは、プレートに播種した細胞や懸濁培養液からペレット化した細胞から培養哺乳類細胞を溶解するのに効果的です。この溶解バッファーはプルダウンおよび免疫沈降アッセイ用に最適化されており、Thermo Scientific Pierce BCAおよび660 nm Protein Assays、タンパク質精製、およびイムノアッセイ（ELISA、ウェスタンブロッティングなど）を含むその他の多くのアプリケーションに使用できます。

Pierce IP Lysis Bufferは25 mMトリス-HCl pH 7.4、150 mM NaCl、1 mM EDTA、1% NP-40、および5%グリセロールで構成されています。このバッファーはプロテアーゼ阻害剤やホスファターゼ阻害剤を含有していませんが、必要に応じて、使用直前にThermo Scientific Halt Protease Inhibitor CocktailまたはPhosphatase Inhibitor Cocktailなどの阻害剤を添加して、タンパク質分解を防止したり、タンパク質のリン酸化を維持できます。

関連製品
M-PER™ Mammalian Protein Extraction Reagent
RIPA Lysis and Extraction Buffer
研究用途にのみご使用ください。診断目的には使用できません。
仕様
フォーマット液体
数量100 mL
容量（メートル法）100 mL
製品ラインPierce
製品タイプIP溶解バッファー
Unit SizeEach
組成および保存条件
受け取り後4℃で保存。

よくあるご質問（FAQ）

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can you provide the shelf-life for the Pierce IP Lysis Buffer?

The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can you provide the shelf life for Pierce IP Lysis Buffer?

Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I store Pierce IP Lysis Buffer (Cat. No. 87787) at room temperature?

Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Which protein assays are compatible with the Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Pierce™ IP Lysis Buffer, 100 mL FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
Rac1 augments Wnt signaling by stimulating ß-catenin-lymphoid enhancer factor-1 complex assembly independent of ß-catenin nuclear import.
Authors:Jamieson C, Lui C, Brocardo MG, Martino-Echarri E, Henderson BR
Journal:
PubMed ID:26403202
ß-Catenin transduces the Wnt signaling pathway and its nuclear accumulation leads to gene transactivation and cancer. Rac1 GTPase is known to stimulate ß-catenin-dependent transcription of Wnt target genes and we confirmed this activity. Here we tested the recent hypothesis that Rac1 augments Wnt signaling by enhancing ß-catenin nuclear import; however, ... More
Characterization of extracellular circulating microRNA.
Authors:Turchinovich A, Weiz L, Langheinz A, Burwinkel B
Journal:Nucleic Acids Res
PubMed ID:21609964
MicroRNAs (miRNAs), a class of post-transcriptional gene expression regulators, have recently been detected in human body fluids, including peripheral blood plasma as extracellular nuclease resistant entities. However, the origin and function of extracellular circulating miRNA remain essentially unknown. Here, we confirmed that circulating mature miRNA in contrast to mRNA or ... More
Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-ß-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity.
Authors:Miyamoto T, Kim D, Knox JA, Johnson E, Mucke L
Journal:J Biol Chem
PubMed ID:26589795
'Diverse lines of evidence suggest that amyloid-ß (Aß) peptides causally contribute to the pathogenesis of Alzheimer disease (AD), the most frequent neurodegenerative disorder. However, the mechanisms by which Aß impairs neuronal functions remain to be fully elucidated. Previous studies showed that soluble Aß oligomers interfere with synaptic functions by depleting ... More