ViewRNA™ Cell Plus Assay Kit

Citations & References (10)

Invitrogen™

ViewRNA™ Cell Plus Assay Kit

製品番号（カタログ番号）	数量
88-19000-99	1 kit

製品番号（カタログ番号） 88-19000-99

価格（JPY）

421,300

お問い合わせください ›

1 kit

ViewRNA Cell Plus Assayは、免疫細胞化学とViewRNA技術、独自の蛍光in situハイブリダイゼーションとシーケンシャル分岐DNA増幅技術を組み合わせた新しいアッセイであり、単一分子感度と個別細胞内のタンパク質を伴うRNAを可視化します。このアッセイでは、免疫細胞化学の間接法および直接法を用いることで、細胞表面または細胞内タンパク質の免疫表現型決定と組み合わせて最大3つのRNAターゲットを同時検出し、特定の細胞サブ集団の詳細な特性評価を行うことが可能です。4番目のRNAターゲットを同時に検出するために、ViewRNA ISH Cell 740 Module（カタログ番号QVC0200）をこのキットと組み合わせることができます。740チャンネル（AlexaFluor 750）の追加ターゲットの解析が可能です。

このViewRNA Cell Plus Assayキットには、アッセイの実施に必要なすべての試薬が含まれています。対象遺伝子および抗体用のターゲットプローブセットは別売りです。

反応性／種
プローブは、あらゆる種に対応できるように設計されています。もっとも高頻度に使用、評価される種は以下のとおりです：アルメリアハムスター、ヒヒ、ウシ、イヌ、ネコ、ニワトリ、チンパネルゼ、ウシ、シノモログスサル、ロバ、ヤギ、ゴールデンシリアンハムスター、モルモット、ハムスター、ウマ、ヒト、サル、サル、マウス、ヒト以外の霊長類、アヌビスヒヒ、ブタ、ブタオザル、ウサギ、ラット、アカゲザル、ヒツジ

報告済みのアプリケーション
顕微鏡、免疫細胞化学

研究用途にのみご使用ください。診断目的には使用できません。

使用対象（アプリケーション）顕微鏡、免疫細胞化学

製品タイプRNA In Situ Hybridization Assay Kit

数量1 kit

検出法プライマープローブ検出

フォーマットキット

Unit SizeEach

よくあるご質問（FAQ）

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To perform ViewRNA assays, which incubator oven should I use, Cat. No. QS0704 or QS0712?

Cat. No. QS0704 is a 120 V unit, for use in US/Canada region (https://www.thermofisher.com/order/catalog/product/QS0704).

Cat. No. QS0712 is a 220 V unit, for use in European region (https://www.thermofisher.com/order/catalog/product/QS0712).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ Cell Plus Assay Kit FAQs

引用および参考文献 (10)

引用および参考文献

Abstract

Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes.

Authors:Rheaume BA, Jereen A, Bolisetty M, Sajid MS, Yang Y, Renna K, Sun L, Robson P, Trakhtenberg EF

Journal:Nat Commun

PubMed ID:30018341

'Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 RGCs (average of 5000 genes per cell) from right and left eyes by single-cell RNA-seq and classify them into 40 ... More

miR-103 promotes endothelial maladaptation by targeting lncWDR59.

Authors:Natarelli L, Geißler C, Csaba G, Wei Y, Zhu M, di Francesco A, Hartmann P, Zimmer R, Schober A

Journal:Nat Commun

PubMed ID:29980665

'Blood flow at arterial bifurcations and curvatures is naturally disturbed. Endothelial cells (ECs) fail to adapt to disturbed flow, which transcriptionally direct ECs toward a maladapted phenotype, characterized by chronic regeneration of injured ECs. MicroRNAs (miRNAs) can regulate EC maladaptation through targeting of protein-coding RNAs. However, long noncoding RNAs (lncRNAs), ... More

Long non-coding RNAs influence the transcriptome in pulmonary arterial hypertension: the role of PAXIP1-AS1.

Authors:Jandl K, Thekkekara Puthenparampil H, Marsh LM, Hoffmann J, Wilhelm J, Veith C, Sinn K, Klepetko W, Olschewski H, Olschewski A, Brock M, Kwapiszewska G

Journal:J Pathol

PubMed ID:30450722

'In idiopathic pulmonary arterial hypertension (IPAH), global transcriptional changes induce a smooth muscle cell phenotype characterised by excessive proliferation, migration, and apoptosis resistance. Long non-coding RNAs (lncRNAs) are key regulators of cellular function. Using a compartment-specific transcriptional profiling approach, we sought to investigate the link between transcriptional reprogramming by lncRNAs ... More

Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry.

Authors:van Buuren N, Kirkegaard K

Journal:Bio Protoc

PubMed ID:30505886

Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can ... More

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Authors:Yang L, Song L, Liu X, Bai L, Li G

Journal:EMBO Rep

PubMed ID:30389724

Despite the success of animal cloning by somatic cell nuclear transfer (SCNT) in many species, the method is limited by its low efficiency. After zygotic genome activation (ZGA) during mouse development, a large number of endogenous retroviruses (ERVs) are expressed, including the murine endogenous retrovirus-L (MuERVL/MERVL). In this study, we ... More

View all ViewRNA™ Cell Plus Assay Kit citations