Nitrocellulose Membranes, 0.2 μm

Name: Nitrocellulose Membranes, 0.2 μm
SKU: 88024

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製品番号（カタログ番号）	Dimensions (LxW)	対応可能
88024	8 cm x 8 cm	15 ミニゲルブロッティング
88013	10.5 cm x 7.9 cm	15 ミニゲルブロッティング
77012	12 cm x 8 cm	25 Mini-gel Blots

3 オプション

製品番号（カタログ番号） 88024

価格（JPY）

32,900

Each

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Dimensions (LxW):

8 cm x 8 cm

対応可能:

15 ミニゲルブロッティング

一括またはカスタム形式をリクエストする

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.2-μm pore size is well-suited for western blotting, and this smaller pore size is ideal for the transfer of low molecular weight proteins or peptides and nucleic acids (<300 bp).

Features include:
100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background—produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

For Research Use Only. Not for use in diagnostic procedures.

仕様

結合能∼209 μg/cm² of protein

概要Nitrocellulose Membranes, 0.2 μm, 8 x 8 cm

数量15シート

出荷条件Room Temperature

Dimensions (LxW)8 cm x 8 cm

フォーマットシート

長さ（メートル法）8 cm

材料100%ニトロセルロースペーパー

孔径0.2 μm

対応可能15 ミニゲルブロッティング

幅（メートル法）8 cm

Unit SizeEach

組成および保存条件

メンブレンは室温で化学蒸気から離れた場所に平らに保存してください。溶媒蒸気の中には、メンブレンを部分的に溶解するものがあり、細孔構造が破壊されることがあります。メンブレンは直射日光の当たらない場所に保管してください。日光はニトロセルロースを乾燥させ、脆くすることがあります。
注：ニトロセルロースメンブレンフィルターは、非常に可燃性が高い製品です。熱や裸火から遠ざけてください。引火点は約 2℃です。メンブレンは 121℃で蒸気オートクレーブにより滅菌できます。

よくあるご質問（FAQ）

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.