Pierce™ HeLa Protein Digest Standard
Pierce™ HeLa Protein Digest Standard
Citations & References (3)
Thermo Scientific™

Pierce™ HeLa Protein Digest Standard

Thermo Scientific Pierce HeLa Protein Digest Standard検証済みの哺乳類タンパク質消化物で、複合プロテオミクスサンプルの質量分析（MS）における品質管理サンプルとして使用できます詳細を見る
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）数量
883295 x 20 μg
8832820 μg
製品番号（カタログ番号） 88329
価格（JPY）
116,600
Each
お問い合わせください ›
数量:
5 x 20 μg
一括またはカスタム形式をリクエストする
Thermo Scientific Pierce HeLa Protein Digest Standard検証済みの哺乳類タンパク質消化物で、複合プロテオミクスサンプルの質量分析（MS）における品質管理サンプルとして使用できます。

HeLa Protein Digest Standardの特長：

•ポジティブコントロールサンプル—HeLa S3細胞株由来の複合哺乳類プロテオームサンプルタンパク質消化物（>15,000タンパク質）
• 完全なトリプシン消化—LysCとトリプシンの両方を使用して調製されているため、トリプシンによる未開裂を10％未満に抑制
• 検証済みのペプチド品質—メチオニン酸化は10％未満、リジンカルバミル化も10％未満
• 厳格に試験済み—ロットごとの分析証明書によって保証および記録された、高品質で一貫性のあるタンパク質消化物
• 安定—安定した凍結乾燥フォーマットで提供

タンパク質消化標準は、確立済みの腺がんリファレンスHeLa S3細胞株由来です。HeLa S3細胞は、関連のある翻訳後修飾により15,000以上のタンパク質を発現するため、複雑なプロテオーム質量分析アプリケーションに最適な標準物質です。細胞ライセートをLysCとトリプシンの両方で消化しているため、トリプシンによる未開裂を最低限に抑え、タンパク質シーケンスカバレッジを向上させています。さらに、Pierce HeLa Protein Digest Standardは他の市販のMS用タンパク質消化物とは異なり、ペプチド品質、消化効率、およびロット間の消化均一性などに関する厳しい品質検査仕様を満たしています。

アプリケーション：
• 定性的LC評価
• LC/MS標準化
• LC/MSメソッド開発
• TMT™試薬メソッド開発

Pierce HeLa Protein Digest Standardは凍結乾燥トリプシンペプチド混合物で、液体クロマトグラフィー（LC）分離、MSメソッド開発、およびMS性能ベンチマーク設定の品質管理標準物質として使用できます。消化物はLC/MS実験用に特別に調製されており、塩や洗剤を含んでいません。複合サンプルの分析前に消化物標準を日常的に使用することで、サンプル間のLC/MS性能を経時的にモニターし、標準化できます。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
最終産物タイプペプチド
使用対象 (装置)質量分析計
数量5 x 20 μg
試薬タイプStandard
ワークフローステップキャリブレーション
検出法質量分析
形状Solid
製品ラインPierce
製品タイプタンパク質消化標準液
原料ペプチド
Unit SizeEach
組成および保存条件
0.0

よくあるご質問（FAQ）

How do I verify the LC-MS system after installation of a new column for proteomic applications?

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I assess my LC-MS system performance?

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Which standards and calibrants do you recommend using for assessing LC-MS system suitability?

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I'm struggling with mass spectrometry sample clean-up and feel that I'm losing peptides. Can you help me verify?

We recommend using the Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) to test your sample clean-up method. Use it directly and also as a control, co-treated with the sample to check for any loss of peptides.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have a low number of protein identifications from my protein digest sample. What can I do?

We recommend checking your mass spectrometry system performance using the Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) to help determine if the problem is from the sample preparation or the LC-MS system.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

引用および参考文献 (3)

引用および参考文献
Abstract
QCloud2: An Improved Cloud-based Quality-Control System for Mass-Spectrometry-based Proteomics Laboratories.
Authors:Olivella R,Chiva C,Serret M,Mancera D,Cozzuto L,Hermoso A,Borràs E,Espadas G,Morales J,Pastor O,Solé A,Ponomarenko J,Sabidó E
Journal:Journal of proteome research
PubMed ID:33724836
QCloud is a cloud-based system to support proteomics laboratories in daily quality assessment using a user-friendly interface, easy setup, and automated data processing. Since its release, QCloud has facilitated automated quality control for proteomics experiments in many laboratories. QCloud provides a quick and effortless evaluation of instrument performance that helps ... More
BoxCar and Library-Free Data-Independent Acquisition Substantially Improve the Depth, Range, and Completeness of Label-Free Quantitative Proteomics.
Authors:Mehta D,Scandola S,Uhrig RG
Journal:Analytical chemistry
PubMed ID:34978796
Data-dependent acquisition (DDA) methods are the current standard for quantitative proteomics in many biological systems. However, DDA preferentially measures highly abundant proteins and generates data that is plagued with missing values, requiring extensive imputation. Here, we demonstrate that library-free BoxCarDIA acquisition, combining MS1-level BoxCar acquisition with MS2-level data-independent acquisition (DIA) ... More
Tailoring to Search Engines: Bottom-Up Proteomics with Collision Energies Optimized for Identification Confidence.
Authors:Révész Á,Milley MG,Nagy K,Szabó D,Kalló G,Csősz É,Vékey K,Drahos L
Journal:Journal of proteome research
PubMed ID:33284634
Bottom-up proteomics relies on identification of peptides from tandem mass spectra, usually via matching against sequence databases. Confidence in a peptide-spectrum match can be characterized by a score value given by the database search engines, and it depends on the information content and the quality of the spectrum. The latter ... More