SILAC(細胞培養におけるアミノ酸を使用した安定的な同位体ラベリング:stable isotope labeling with amino acids in cell culture)は、複雑なタンパク質サンプルにおける相対的な差異変化を同定および定量するための強力な方法です。このアプローチでは、タンパク質の迅速かつ包括的な同定、特性評価、定量のために、重い13C-または15N-標識アミノ酸をタンパク質に生体外代謝組み込みを行い、質量分析(MS)を行います。
Do you offer any heavy amino acids other than the ones in the SILAC Protein Quantitation Kits?
We offer additional isotopes of lysine, including 4,4,5,5 D4 L-Lysine-2HCl (Cat. No. 88437) and 13C6 15N2 L-Lysine-2HCl (Cat. No. 88209), which can be used for multiplexed experiments. 13C6 L-Arginine-HCl (Cat. No. 88210) and 13C6 15N2 L-Arginine-HCl (Cat. No. 89990) are also available to enhance peptide isotope label coverage of tryptic peptides. In addition to lysine and arginine, 13C6 L-Leucine (Cat. No. 88435) can also be used with our powdered SILAC DMEM (Cat. No. 88425) and RPMI-1640 media (Cat. No. 88426). Please check the product page for a current listing.