Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL
Citations & References (3)
Thermo Scientific™

Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL

Thermo Scientific™ Pierce™タンパク質コンセントレータPESは、生物学的サンプルの濃縮、脱塩、緩衝液交換用のポリエーテルスルホン（PES）膜を備えた使い捨て限外ろ過遠心装置です。この製品は、分子量カットオフ詳細を見る
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製品番号（カタログ番号）数量サンプル量
8852824濃縮装置5 to 20 mL
8851325濃縮装置100 to 500 μL
8851610反応2 to 6 mL
8851724濃縮装置2 to 6 mL
8852710反応5 to 20 mL
885354濃縮装置20 to 100 mL
製品番号（カタログ番号） 88528
価格（JPY）
48,000
Each
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数量:
24濃縮装置
サンプル量:
5 to 20 mL
Thermo Scientific™ Pierce™タンパク質コンセントレータPESは、生物学的サンプルの濃縮、脱塩、緩衝液交換用のポリエーテルスルホン（PES）膜を備えた使い捨て限外ろ過遠心装置です。この製品は、分子量カットオフ（MWCO）が10K で、5～20 mLのサンプル量の処理に使用できます。

Pierce Protein Concentrators PESの特長：

•迅速なタンパク質濃縮—10～30倍のサンプル濃度は、5～30分で達成できます（10K MWCOの場合。他のMWCOでは時間が異なる場合あり）
•優れたタンパク質回収—一般的なタンパク質回収は>90%
•汎用性—生物学的サンプルの濃縮、透析ろ過、緩衝液交換を実施
•互換性—固定角ローターとスイング式ブッカーローターの両方で使用可能。逆回転を行わずにサンプルを収集および回収

容易に識別できるように、MWCO値は各装置の側面にエッチングされています。サンプルの添加と回収を容易にするため、各装置の側面に目盛り付きの透明なウィンドウが設けられており、残余量を視覚的に測定できます。膜の垂直設計により、膜上での高いフラックス率、低い非特異的結合、およびわずかなタンパク質凝集が可能になり、信頼性と一貫性のある結果が得られます。

Pierce Protein Concentrators PESには、装置内PES膜のMWCO定格の2倍以上の分子量を持つ分子を保持するための膜が含まれています。推奨質量よりも小さい分子を持つ濃縮装置を使用すると、回収率が低下する場合があります。タンパク質回収率は、サンプル中の特定のタンパク質とその出発濃度によって異なります。タンパク質の回収率を>90%超にするには、最小タンパク質サンプル濃度を0.1 mg/mLにする必要があります。
For Research Use Only. Not for use in diagnostic procedures.
仕様
概要50 mLコニカル、スピンカラム
製品ラインPierce
精製標的タンパク質
数量24濃縮装置
出荷条件室温
タイプフィルター
ディスポーザブル可
MWCO10 kDa
材料（膜）PES
サンプル量5 to 20 mL
Unit SizeEach
組成および保存条件
室温保存。

よくあるご質問（FAQ）

Can I autoclave PES protein concentrators?

We do not recommend autoclaving the PES protein concentrators. High temperatures will significantly increase the membrane molecular weight cutoff (MWCO). To sterilize, we recommend using a 70% ethanol solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use PES protein concentrators for desalting and buffer exchange?

Yes, the PES membrane is compatible with desalting and buffer exchange. Protocols for concentrating, desalting, and buffer exchange only are provided in the product manuals.

How do PES protein concentrators work?

The protein concentrator columns have an upper compartment that holds the sample, and a lower compartment. The two compartments are separated by a polyethersulfone (PES) semipermeable membrane. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The protein concentrator cassettes also contain a PES membrane and a unique channel design that ensures high optimal flux with no optimization effort. Unlike other systems, the fluid is passed parallel to the filter, rather than being pushed through a membrane perpendicularly, which can clog the filter. For maximum protein recovery in both columns and cassettes, samples should have a molecular weight two-fold greater than the molecular weight cutoff (MWCO) of the concentrator membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the different sizes of PES protein concentrators you offer?

Pierce polyethersulfone (PES) protein concentrators are offered in six distinct MWCOs of 3K, 5k, 10K, 30K, 50K, and 100K, and the cassettes are offered in five membrane MWCOs of 3K, 10K, 30K, 50K, and 100K. The columns can process four different sample volumes ranges of 100-500 µL, 2-6 mL, 5-20 mL, and 20-100 mL, while the cassettes can process 0.5-6 L. Please see our selection chart and video found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/protein-concentrators.html).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I labeled my protein with your kit, but now it‘s too dilute for my application. What should I do?

There are different ways to concentrate your protein. These include:

Centrifugation (filtration) using concentrators
Dialysis
Lyophilization
Precipitation

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
Scalable spatial single-cell transcriptomics and translatomics in 3D thick tissue blocks.
Authors:Sui X,Lo JA,Luo S,He Y,Tang Z,Lin Z,Zhou Y,Wang WX,Liu J,Wang X
Journal:bioRxiv : the preprint server for biology
PubMed ID:39149316
Characterizing the transcriptional and translational gene expression patterns at the single-cell level within their three-dimensional (3D) tissue context is essential for revealing how genes shape tissue structure and function in health and disease. However, most existing spatial profiling techniques are limited to 5-20 μm thin tissue sections. Here, we developed ... More
Phages carry orphan antitoxin-like enzymes to neutralize the DarTG1 toxin-antitoxin defense system.
Authors:Johannesman A,Carlson NA,LeRoux M
Journal:bioRxiv : the preprint server for biology
PubMed ID:39026772
The astounding number of anti-phage defenses encoded by bacteria is countered by an elaborate set of phage counter-defenses, though their evolutionary origins are often unknown. Here, we discover an orphan antitoxin counter-defense element in T4-like phages that can overcome the bacterial toxin-antitoxin phage defense system, DarTG1. The DarT1 toxin, an ... More
Keratinocyte-Derived Exosomes in Painful Diabetic Neuropathy.
Authors:Coy-Dibley J,Jayaraj ND,Ren D,Pacifico P,Belmadani A,Wang YZ,Gebis KK,Savas JN,Paller AS,Miller RJ,Menichella DM
Journal:bioRxiv : the preprint server for biology
PubMed ID:39229068
Painful diabetic neuropathy (PDN) is a challenging complication of diabetes with patients experiencing a painful and burning sensation in their extremities. Existing treatments provide limited relief without addressing the underlying mechanisms of the disease. PDN involves the gradual degeneration of nerve fibers in the skin. Keratinocytes, the most abundant epidermal ... More