Pierce™ Protein A/G Magnetic Beads
Pierce™ Protein A/G Magnetic Beads
Citations & References (11)
Thermo Scientific™

Pierce™ Protein A/G Magnetic Beads

Thermo Scientific Pierce Protein A/G Magnetic Beadsは、手動またはロボット磁気分離機を使用した抗体精製や免疫沈降法用の高性能アフィニティー粒子です。Protein A/G詳細を見る
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製品番号（カタログ番号）数量
888021 mL
888035 mL
製品番号（カタログ番号） 88802
価格（JPY）
42,900
Each
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数量:
1 mL
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Thermo Scientific Pierce Protein A/G Magnetic Beadsは、手動またはロボット磁気分離機を使用した抗体精製や免疫沈降法用の高性能アフィニティー粒子です。

Protein A/G Magnetic Beadsの特長：

•高結合能—結合能は他のサプライヤーの一般的な磁気ビーズより約4倍高いため、実験あたりの使用量が低減
• 低い非特異的結合—安定したブロック済みビーズは高純度の産物を提供（たとえば、抗体による免疫沈降（IP）で溶出した抗原は、複雑なIPマトリックス由来の汚染タンパク質を含まない）
• 柔軟性—Protein AとProtein Gの両方のIgG結合ドメインを1つのビーズに組み合わせた利便性
• 互換性—ビーズは手動および自動化アプリケーション（Thermo Scientific KingFisher装置など）に対応
• アッセイの一貫性—磁気ビーズではレジン損失がなく、微量遠心分離チューブのみを使用する従来のIP法より効率的な溶液の分離が可能

これらの磁気ビーズは、Protein AとProtein Gの両方のIgG結合ドメインを結合する組換え融合タンパク質である遺伝子組換えPierce Protein A/Gでコーティングされています。これにより、いずれかのタンパク質単独よりもより幅広い種やアイソタイプから抗体を捕捉できます。当社のクロスリンカーを使用して、抗体を磁気粒子に固定し、免疫沈降サンプルのIgG汚染を防止きます。これらのビーズは、磁気スタンドで手動で使用することも、Thermo Scientific KingFisher装置などの自動化プラットフォームでも使用できます。

アプリケーション：
• IPおよびCo-IP実験（完全キットを参照）
• 非還元条件での分析用免疫沈降
• 抗体精製

Pierce Magnetic Beadsに固定された組換えProtein A/Gは、Protein AとProtein Gの両方のIgG結合ドメインを融合したものです。Protein A/GはProtein Aから4つの、Protein Gから2つのFc結合ドメインを含むため、イムノグロブリンの研究や精製に便利なツールです。したがって、Pierce Magnetic Particlesは個別のProtein AとProtein Gポリペプチドの混合を固定したものではなく、Protein A磁気ビーズとProtein G磁気ビーズの混合物でもありません。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
濃度Slurry: 10mg/mL, 1% solids
リガンドタイプタンパク質A/G
タンパク質形態Recombinant
数量1 mL
ターゲット抗体
容量（メートル法）1 mL
形状Liquid
粒子径1 μm
製品ラインPierce
タイプ磁気ビーズ
Unit SizeEach
組成および保存条件
受け取り後4℃で保存。製品はアイスパック入りで出荷されます。

よくあるご質問（FAQ）

Can I reuse Pierce Protein A/G Magnetic Beads (Cat. No. 88802, 88803)?

Pierce Protein A/G Magnetic Beads are intended for single use only. Therefore, we do not recommend reusing them.

What advantage do Pierce Magnetic Agarose beads have over Dynabeads or Pierce Magnetic Beads?

Pierce Magentic Agarose Beads have a much higher binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I centrifuge the Thermo Scientific Protein A/G Magnetic Beads?

No, centrifuging causes the formation of irreversible aggregates which greatly reduces binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I reduce non-specific binding to Thermo Scientific Protein A/G Magnetic Beads?

Adding 50-350 mM of NaCl to the Binding/Wash and Elution Buffers can help reduce non-specific bands. Also, use a low-pH elution for single-use applications. The optimal time for low-pH elution is 10 minutes; exceeding 10 minutes may result in non-specific binding and yield reduction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I avoid boiling the Thermo Scientific Protein A/G Magnetic Beads?

Avoid bead-boil elutions when using rabbit antibodies (primary or secondary) in downstream Western blot applications. Instead, perform elution in SDS-PAGE sample buffer at room temperature. For all other antibody species, boiling the beads in SDS-PAGE sample buffer is acceptable for single-use applications. Boiling could cause bead aggregation and loss of binding activity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ Protein A/G Magnetic Beads FAQs

引用および参考文献 (11)

引用および参考文献
Abstract
The Thyroid Hormone Analog GC-1 Mitigates Acute Lung Injury by Inhibiting M1 Macrophage Polarization.
Authors:Li B,Xia C,He W,Liu J,Duan R,Ji Z,Pan X,Zhou Y,Yu G,Wang L
Journal:Advanced science (Weinheim, Baden-Wurttemberg, Germany)
PubMed ID:39373388
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a life-threatening condition with a high mortality rate of ≈40%. Thyroid hormones (THs) play crucial roles in maintaining homeostasis of the cellular microenvironment under stress. The previous studies confirmed that the clinical-stage TH analog GC-1 significantly alleviates pulmonary fibrosis by improving ... More
E3 ubiquitination ligase XIAP lightens diabetes-induced cognitive impairment by inactivating TXNIP-ERS-mediated neuronal injury.
Authors:Zhang Q,Huang HJ,Zhang JL,Tian Y,Hu Y
Journal:The Kaohsiung journal of medical sciences
PubMed ID:39629879
Diabetes-induced cognitive dysfunction (DCD) is a neurological disorder associated with diabetes, characterized by cognitive impairment driven by neuronal injury from chronic high glucose (HG) exposure. This study aims to elucidate the role and mechanisms of the X-linked inhibitor of apoptosis protein (XIAP)/thioredoxin-interacting protein (TXNIP) in hippocampal neuron cell death and ... More
Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure.
Authors:Mercier T,Bouvet M,Dubois-Deruy E,Dechaumes A,Beseme O,Richard V,Mulder P,Pinet F
Journal:Frontiers in endocrinology
PubMed ID:30344511
Post-translational modifications (PTMs) of sarcomeric proteins could participate to left ventricular (LV) remodeling and contractile dysfunction leading in advanced heart failure (HF) with altered ejection fraction. Using an experimental rat model of HF (ligation of left coronary artery) and phosphoproteomic analysis, we identified an increase of desmin phosphorylation and a ... More
Extracellular matrix stiffness regulates degradation of MST2 via SCF (βTrCP).
Authors:Fiore APZP,Rodrigues AM,Ribeiro-Filho HV,Manucci AC,de Freitas Ribeiro P,Botelho MCS,Vogel C,Lopes-de-Oliveira PS,Pagano M,Bruni-Cardoso A
Journal:Biochimica et biophysica acta. General subjects
PubMed ID:36044955
The Hippo pathway plays central roles in relaying mechanical signals during development and tumorigenesis, but how the proteostasis of the Hippo kinase MST2 is regulated remains unknown. Here, we found that chemical inhibition of proteasomal proteolysis resulted in increased levels of MST2 in human breast epithelial cells. MST2 binds SCF(βTrCP) ... More
Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure.
Authors:Carreon TA,Castellanos A,Gasull X,Bhattacharya SK
Journal:Scientific reports
PubMed ID:28352076
In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) protein cochlin with the cell surface bound and ... More
View all Pierce™ Protein A/G Magnetic Beads citations