Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Citations & References (12)
Thermo Scientific™

Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

Thermo Scientific Zebaスピン脱塩プレート（7K MWCO）には、2 µL～4 mLのタンパク質サンプルの遠心分離で優れた脱塩性能を発揮するサイズ排除クロマトグラフィーレジンが充填されています。Zebaスピン脱塩プレートの特長：•高い回収率詳細を見る
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製品番号（カタログ番号）数量フォーマット容量（メートル法）
8988225カラム微量遠心機、スピンカラム0.5 mL
8987725カラム微量遠心機、スピンカラム75 μL
8987850カラム微量遠心機、スピンカラム75 μL
A400024695カラムSpin Column20 mL
A4000247510 ColumnsSpin Column20 mL
8989025カラム15 mLコニカル、スピンカラム2 mL
898915カラム15 mLコニカル、スピンカラム5 mL
8989225カラム15 mLコニカル、スピンカラム5 mL
898935カラム50 mLコニカル、スピンカラム10 mL
8989425カラム50 mLコニカル、スピンカラム10 mL
898895カラム15 mLコニカル、スピンカラム2 mL
8988350カラム微量遠心機、スピンカラム0.5 mL
899345カラムクロマトグラフィーカートリッジ1 mL
899355カラムクロマトグラフィーカートリッジ5 mL
89807プレート2枚96-ウェルフィルタープレート96-well
89808プレート4枚96-ウェルフィルタープレート96-well
製品番号（カタログ番号） 89882
価格（JPY）
34,300
Each
お問い合わせください ›
数量:
25カラム
フォーマット:
微量遠心機、スピンカラム
容量（メートル法）:
0.5 mL
一括またはカスタム形式をリクエストする
Thermo Scientific Zebaスピン脱塩プレート（7K MWCO）には、2 µL～4 mLのタンパク質サンプルの遠心分離で優れた脱塩性能を発揮するサイズ排除クロマトグラフィーレジンが充填されています。

Zebaスピン脱塩プレートの特長：

•高い回収率 — 低結合性レジンでタンパク質の回収率を最大化します
• 高速 — 分画のスクリーニングが不要。重力フローによるタンパク質出現を待つこともありません
• 経済的 — 他の市販のカートリッジよりも低コストで優れた性能を発揮します

Zeba脱塩プレートは、高性能のZeba脱塩レジンを含有するポリプロピレン製装置で、迅速で優れたタンパク質脱塩を行うとともに、7,000ダルトンを超えるタンパク質を高率で回収します。Zeba脱塩装置は、2 µL～4 mLのサンプルの脱塩とバッファー交換を簡単に実行できます。この96ウェルスピンプレートフォーマットにより、よりハイスループットな処理が可能になります。

Zeba 96ウェルスピン脱塩プレートは、レジンの分注や膨潤が不要で、スピンカラムと同等の高タンパク質回収率を実現し、塩や他の低分子を95%以上除去します。1枚のプレートで96個の少量サンプル（20～100 µL）を5分で処理できます。

詳細な製品データ
• Zebaスピン脱塩カラムを用いたベンチマーク実験
• 脱塩精製、アフィニティー精製のためのクロマトグラフィーカートリッジ

関連製品
Zeba™ マイクロスピン脱塩カラム、7K MWCO、75 µL
Zeba™スピン脱塩カラム、7K MWCO、0.5 mL
Zeba™スピン脱塩カラム、7K MWCO、2 mL
Zeba™スピン脱塩カラム、7 K MWCO、5 mL
Zeba™スピン脱塩カラム、7 K MWCO、10 mL
For Research Use Only. Not for use in diagnostic procedures.
仕様
製品タイプスピン脱塩カラム
精製標的緩衝液交換、タンパク質
数量25カラム
容量（メートル法）0.5 mL
カラムタイプサイズ排除、特許取得済みレジン
フォーマット微量遠心機、スピンカラム
MWCO7.0 kDa
製品ラインZeba
サンプル量（メートル法）30 to 130 μL
Unit SizeEach
組成および保存条件
受け取り後4℃で保存。

よくあるご質問（FAQ）

Besides precipitation with ammonium sulfate, what are some ways I can concentrate my protein sample?

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Desalting columns, plates, and cartridges be autoclaved?

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

How should I store Zeba Spin Desalting Columns, Plates, and Cartridges?

We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What product do you recommend for removing sodium azide from the Alix Monoclonal Antibody (3A9) (Cat. No. MA1-83977) storage solution?

We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL FAQs

引用および参考文献 (12)

引用および参考文献
Abstract
Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosis.
Authors:Yuan F,Gollapudi S,Day KJ,Ashby G,Sangani A,Malady BT,Wang L,Lafer EM,Huibregtse JM,Stachowiak JC
Journal:PNAS nexus
PubMed ID:39253396
Clathrin-mediated endocytosis is an essential cellular pathway that enables signaling and recycling of transmembrane proteins and lipids. During endocytosis, dozens of cytosolic proteins come together at the plasma membrane, assembling into a highly interconnected network that drives endocytic vesicle biogenesis. Recently, multiple groups have reported that early endocytic proteins form ... More
Microscale Thermophoresis (MST) as a Tool to Study Binding Interactions of Oxygen-Sensitive Biohybrids.
Authors:Jagilinki BP,Willis MA,Mus F,Sharma R,Pellows LM,Mulder DW,Yang ZY,Seefeldt LC,King PW,Dukovic G,Peters JW
Journal:Bio-protocol
PubMed ID:39131194
Microscale thermophoresis (MST) is a technique used to measure the strength of molecular interactions. MST is a thermophoretic-based technique that monitors the change in fluorescence associated with the movement of fluorescent-labeled molecules in response to a temperature gradient triggered by an IR LASER. MST has advantages over other approaches for ... More
Enabling the formation of native mAb, Fab' and Fc-conjugates using a bis-disulfide bridging reagent to achieve tunable payload-to-antibody ratios (PARs).
Authors:Thoreau F,Rochet LNC,Baker JR,Chudasama V
Journal:Chemical science
PubMed ID:37035695
Either as full IgGs or as fragments (Fabs, Fc, etc.), antibodies have received tremendous attention in the development of new therapeutics such as antibody-drug conjugates (ADCs). The production of ADCs involves the grafting of active payloads onto an antibody, which is generally enabled by the site-selective modification of native or ... More
Reconstitution of the DTX3L-PARP9 complex reveals determinants for high-affinity heterodimerization and multimeric assembly.
Authors:Ashok Y,Vela-Rodríguez C,Yang C,Alanen HI,Liu F,Paschal BM,Lehtiö L
Journal:The Biochemical journal
PubMed ID:35037691
Ubiquitination and ADP-ribosylation are post-translational modifications that play major roles in pathways including the DNA damage response and viral infection. The enzymes responsible for these modifications are therefore potential targets for therapeutic intervention. DTX3L is an E3 Ubiquitin ligase that forms a heterodimer with PARP9. In addition to its ubiquitin ... More
Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits.
Authors:Grove DJ,Levine DJ,Kearse MG
Journal:Nucleic acids research
PubMed ID:37602404
eIF2A was the first eukaryotic initiator tRNA carrier discovered but its exact function has remained enigmatic. Uncharacteristic of translation initiation factors, eIF2A is reported to be non-cytosolic in multiple human cancer cell lines. Attempts to study eIF2A mechanistically have been limited by the inability to achieve high yield of soluble ... More
View all Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL citations