What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?
Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Can Zeba Desalting Chromatography Cartridges be regenerated and stored after use?
Yes, Zeba Desalting Chromatography Cartridges can be regenerated and stored for later use. The catridges may be reused multiple times without significant loss of performance.
For regeneration, wash the resin with 3-5 column volumes of water or buffer before applying the next sample. For storage, wash the resin with 5 column volumes of 0.02-0.05% sodium azide in water. Attach the supplied bottom cap and then the top plug and store the cartridge at 4 degrees C.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
What product do you recommend for removing sodium azide from the Alix Monoclonal Antibody (3A9) (Cat. No. MA1-83977) storage solution?
We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.