FlashTag™ Biotin HSR RNA Labeling Kits

The ERCC controls are not tiled onto GeneChip miRNA arrays because they would not work with the miRNA array/assay system. The FlashTag Biotin HSR RNA Labeling Kits used to label miRNAs is optimized for shortRNAs. Even though longer mRNAs like the ERCC controls will be labeled, the HWS (hyb/wash/stain) conditions are not optimal and consequently, the longer mRNAs typically do not get detected.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Due to certain miRNAs being conserved across species, there may be multiple copies of the same probe across the miRNA arrays. However, sample processing with the FlashTag Biotin HSR RNA Labeling Kits ensures that there is excess target compared to the probes on the array. Consequently, there is enough sample available to hybridize onto all the replicate probes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

In order to perform RNA isolation, any of our kits for purification of total RNA or LMW RNA will be compatible with the FlashTag Biotin HSR RNA Labeling Kits.We also recommend that you elute or resuspend the RNA in nuclease-free water to ensure that the purification method retains low mole.

Reverse transcribed miRNA would not work because it would be the wrong sense (antisense, while our probes interrogate sense RNA). Amplified miRNA should work, but the miRNAs are very short. Therefore, adapters may need to be ligated to them or tailing may be needed to get enough runway to complete amplification. It is unknown if any extra sequence may interfere with the hybridization.

If you are using a FlashTag Biotin HSR RNA Labeling Kit for the GeneChip miRNA 3.1 Array Strip, we recommend staying within the range of 130-1000 ng input total RNA.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.