Black 96-Well Immuno Plates

For energy treated plates, the entire plate surface receives the energy treatment. The MaxiSorp, PolySorp, MediSorp and MultiSorp plates are energy treated plates. For traditionally treated plates, the well surface is treated up to the listed working volume.

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

Opaque plates can only be used on “top reader” instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Black plates are often used in assays that make use of a fluorescent reporter because the opaque black surface effectively isolates the signal from each well in order to eliminate “crosstalk” or signal bleed. White plates also isolate well-to-well signals somewhat, but also provide a reflective background to direct luminescent output to the reader, and so often serve as a better choice to improve dynamic range from a luminescent reporter.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.