StemPro™ Chondrogenesis Differentiation Kit

Citations & References (2)

Gibco™

StemPro™ Chondrogenesis Differentiation Kit

製品番号（カタログ番号）	数量
A1007101	1 Kit

製品番号（カタログ番号） A1007101

価格（JPY）

53,500

お問い合わせください ›

1 Kit

StemPro™軟骨形成分化キットは、間葉系幹細胞（MSC）を組織培養容器内で軟骨形成分化する目的で開発されました。このキットには、軟骨形成経路にMSCを誘導し、軟骨細胞を生成するために必要なすべての試薬が含まれています。StemPro™軟骨形成分化キットをStemPro™ MSC SFMまたはMesenPRO RS™ Mediumと組み合わせて使用することで、MSCの単離、増殖、コラーゲン基質産生軟骨細胞への分化のための標準化された培養ワークフローソリューションが実現します。

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプ幹細胞（間葉系）

培養環境CO2

エンドトキシンレベル≦50 EU/mL、≦50 EU/ml

フォーマット液体

グルコース低グルコース

グルタミンGlutaMAX™-I

HEPESバッファーHEPESなし

フェノールレッド指示薬フェノールレッドなし

純度または品質グレード研究グレード

数量1 Kit

研究カテゴリー分化

血清レベル血清フリー

炭酸水素ナトリウムバッファー重炭酸ナトリウム

ピルビン酸ナトリウム添加剤ピルビン酸ナトリウム

使用対象（アプリケーション）幹細胞の分化

製品ラインGibco、StemPro

製品タイプChondrogenesis Differentiation Kit

Unit SizeEach

組成および保存条件

このキットには、100 mlの完全な培地を調製するのに適した100 mlの基本培地と10 mlの増殖サプリメントが付属しています。各コンポーネントには、それぞれの輸送要件および保管要件が伴います。増殖サプリメントは個別に販売されていません。
StemPro™骨細胞／軟骨細胞分化用基礎培地：2～8℃（光から保護）
StemPro™軟骨形成サプリメント：-5～-20℃（暗所）

よくあるご質問（FAQ）

How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What mesenchymal stem cells (MSC) sources can I use with the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

The StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101) was designed and tested using human adipose-derived stem cells (ADSC). Based on protocols in literature, it should also work with mesenchymal stem cells (MSC) from other sources, including umbilical cord and cord blood.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The StemPro Chondrogenesis Differentiation Kit manual refers to MSC Attachment Medium under Chondrogenesis Differentiation, Step 7. Is MSC Attachment Medium the same as MSC growth medium?

Yes, MSC Attachment Medium is the same as MSC growth medium.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

View all StemPro™ Chondrogenesis Differentiation Kit FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Mice lacking Nf1 in osteochondroprogenitor cells display skeletal dysplasia similar to patients with neurofibromatosis type I.

Authors:Wang W, Nyman JS, Ono K, Stevenson DA, Yang X, Elefteriou F,

Journal:Hum Mol Genet

PubMed ID:21757497

Mutations in NF1 cause neurofibromatosis type I (NF1), a disorder characterized, among other clinical manifestations, by generalized and focal bony lesions. Dystrophic scoliosis and tibial pseudoarthrosis are the most severe skeletal manifestations for which treatment is not satisfactory, emphasizing the dearth of knowledge related to the biology of NF1 in ... More

Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.

Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,

Journal:Tissue Eng Part C Methods

PubMed ID:30412046

cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More