StemPro™ Osteogenesis Differentiation Kit

Citations & References (3)

Gibco™

StemPro™ Osteogenesis Differentiation Kit

製品番号（カタログ番号）	数量
A1007201	1 Kit

製品番号（カタログ番号） A1007201

価格（JPY）

53,600

お問い合わせください ›

1 Kit

ヒト間葉系幹細胞から骨細胞への完全な分化に対応

StemPro™骨形成分化キットは、間葉系幹細胞（MSC）を組織培養容器内で骨形成分化する目的で開発されました。このキットには、骨形成経路にMSCを誘導し、未成熟骨細胞および成熟骨細胞を生成するために必要な
すべての試薬が含まれています。StemPro™骨形成分化キットをMesenPRO RS™培地またはStemPro™ MSC SFMと組み合わせて使用することで、MSCの単離、増殖、および石灰化した基質産生骨細胞への分化のための標準化された培養ワークフローソリューションが実現します。

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプ幹細胞（間葉系）

培養環境CO2

エンドトキシンレベル≦50 EU/mL、≦50 EU/ml

フォーマット液体

グルコース低グルコース

グルコース濃度1,000 mg/L

グルタミンGlutaMAX™-I

HEPESバッファーHEPESなし

無機塩類リン酸ナトリウム

フェノールレッド指示薬フェノールレッドなし

純度または品質グレード研究グレード

数量1 Kit

研究カテゴリー分化

血清レベル標準血清

炭酸水素ナトリウムバッファー重炭酸ナトリウム

ピルビン酸ナトリウム添加剤ピルビン酸ナトリウム

サプリメント骨形成サプリメント

使用対象（アプリケーション）幹細胞の分化

製品ラインGibco、StemPro

製品タイプOsteogenesis Differentiation Kit

Unit SizeEach

組成および保存条件

このキットは、別々の保存要件を持つ2つの部品にして出荷します。これらのコンポーネントは個別には販売されていません。
•StemPro™骨細胞／軟骨細胞分化用基礎培地（100 ml、2℃～8℃の暗所で保存）
• StemPro™骨形成サプリメント（10 ml、-5℃～-20℃の暗所で保存）

基礎培地は室温で出荷され、サプリメントはドライアイスで出荷されます。

よくあるご質問（FAQ）

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

引用および参考文献 (3)

引用および参考文献

Abstract

Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.

Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,

Journal:

PubMed ID:26564250

'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More

Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells.

Authors:Swioklo S, Constantinescu A, Connon CJ,

Journal:Stem Cells Transl Med

PubMed ID:26826163

: Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C-23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium ... More

Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.

Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,

Journal:Tissue Eng Part C Methods

PubMed ID:30412046

cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More