CELLstart™ Substrate
CELLstart™ Substrate
Citations & References (3)
Gibco™

CELLstart™ Substrate

CELLstart Substrate is a defined substrate that supports human embryonic stem cell (hESC) attachment and expansion of undifferentiated colonies in詳細を見る
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製品番号（カタログ番号）数量
A10142012 mL
製品番号（カタログ番号） A1014201
価格（JPY）
56,700
Each
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数量:
2 mL

CELLstart Substrate is a defined substrate that supports human embryonic stem cell (hESC) attachment and expansion of undifferentiated colonies in serum-free medium without the need for feeder cells. It can also be used with mesenchymal and neural stem cells and for the attachment of human feeder cells (e.g., foreskin fibroblasts).

  • Defined substrate for stem cell culture
  • cGMP manufactured to help ensure manufacturability and traceability

GIBCO products are of high quality and are supplied with harmonized documentation such as certificates of analysis and certificates of origin.

研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプ幹細胞(神経)、幹細胞(iPS-人工多能性幹)、幹細胞(胚性)、幹細胞(間葉系)、幹細胞(間葉系)
分類血清フリー
エンドトキシンレベル≦50 EU/mL、≦ 50 EU/mL
フォーマットチューブ
フェノールレッド指示薬フェノールレッドなし
製品ラインGibco
純度または品質グレードhESC適格性確認済み
数量2 mL
出荷条件室温
形状液体
Unit SizeEach
組成および保存条件
2~8°Cにて保存してください。

よくあるご質問（FAQ）

How do I prepare the StemPRO hESC SFM complete medium?

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used to culture mouse ESCs?

No, we don't recommend StemPro hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut DMEM and KnockOut SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used for new cell line derivations?

Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell lines has StemPRO hESC SFM been tested with?

StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemPRO hESC SFM require the use of a basement membrane matrix?

Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CELLstart™ Substrate FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice.
Authors:Haus DL, Nguyen HX, Gold EM, Kamei N, Perez H, Moore HD, Anderson AJ, Cummings BJ
Journal:
PubMed ID:25082219
'Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient expansion of resulting hNSCs for scaling out or up ... More
Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.
Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,
Journal:
PubMed ID:26564250
'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More
Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.
Authors:Swistowski A, Peng J, Han Y, Swistowska AM, Rao MS, Zeng X,
Journal:PLoS One
PubMed ID:19597550
BACKGROUND: Human embryonic stem cells (hESCs) may provide an invaluable resource for regenerative medicine. To move hESCs towards the clinic it is important that cells with therapeutic potential be reproducibly generated under completely defined conditions. METHODOLOGY/PRINCIPAL FINDINGS: Here we report a four-step scalable process that is readily transferable to a ... More