How do the following compounds help cells attach in culture - collagen, gelatin, hydrated collagen, matrigel, poly-L-ornithine, poly-L-lysine, and poly-D-lysine?
The polystyrene that culture flasks are made of tends to have a negative charge. Many cell types will still attach because the plasticware is treated in a way to make them hydrophilic. However cell types that do not attach well to the plastic, or are grown in medium with little or no serum protein, will attach better if the plastic is coated with a protein/polymer which has an overall positive charge.
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Is the Collagen I, rat tail (Cat. No. A1048301) pepsin-digested?
No, the Collagen I, rat tail is not pepsin-digested.
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Do you offer an alternative product for Collagen I, rat tail (Cat. No. A1048301), that can be prepared as a clear liquid gel for coating tissue culture plates?
An alternative for Collagen I, rat tail (Cat. No A1048301) is Collagen I, bovine (Cat. No A1064401). While the source of Collagen I is different, they can both be prepared as a clear gel providing a 3D matrix or surface coating on tissue culture plates as a substrate for culturing primary cells such as keratinocytes and hepatocytes.
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I would like to use Collagen I Rat Protein, Tail (Cat. No. A1048301) for coating cell culture dishes. In the first step of the thin coating protocol, what should I use to dilute the 17.4 M acetic acid stock solution to 20 mM?
You can just use water to dilute the acetic acid stock solution to 20 mM.
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