Modified IMEM (Improved Minimum Essential Medium), with L-glutamine, without gentamicin sulfate

Gibco™

Modified IMEM (Improved Minimum Essential Medium), with L-glutamine, without gentamicin sulfate

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used詳細を見る

製品番号（カタログ番号）	数量
A1048901	500 mL

製品番号（カタログ番号） A1048901

価格（JPY）

7,400

お問い合わせください ›

500 mL

Customize this product

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With	Without
L-glutamine	Gentamycin
Proline	HEPES
Sodium Pyruvate	Insulin
Zinc
Phenol Red

The complete formulation is available.

Gibco™ Richter's MEM is a modified formulation of Eagle's MEM that contains iron, zinc, putrescine, fatty acids, and gentamycin. Richter's modification also includes very high concentrations of both choline chloride and i-Inositol. This product was further modified with L-glutamine, and without gentamicin sulfate.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Gibco™ MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

研究用途にのみご使用ください。診断目的には使用できません。

細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

細胞タイプPrimary Rat Astrocytes

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプMEM (Minimum Essential Medium)

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

無菌性Sterile-filtered

添加剤ありLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Zinc

添加剤なしNo Gentamicin, No HEPES

Unit SizeEach

組成および保存条件

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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