MEM α, nucleosides, no ascorbic acid

Citations & References (1)

Gibco™

MEM α, nucleosides, no ascorbic acid

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative詳細を見る

製品番号（カタログ番号）	数量
A1049001	500 mL

製品番号（カタログ番号） A1049001

価格（JPY）

15,000

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500 mL

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MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With	Without
Deoxyribonucleosides	Ascorbic acid
Ribonucleosides
L-glutamine
Phenol Red

The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

研究用途にのみご使用ください。診断目的には使用できません。

細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

細胞タイプPrimary Rat Astrocytes

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプMEM α (Minimum Essential Medium α)

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

添加剤ありLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides

添加剤なしNo Ascorbic Acid

Unit SizeEach

組成および保存条件

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM α, nucleosides, no ascorbic acid FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Osteopontin increases breast cancer cell sensitivity to specific signaling pathway inhibitors in preclinical models.

Authors:Mutrie JC, Chambers AF, Tuck AB

Journal:Cancer Biol Ther

PubMed ID:21795853

Src kinase and Hsp90 play important roles in malignancy-promoting signaling pathways in a variety of cancers and related targeting agents are presently in clinical trials. To help improve the success of Src kinase and Hsp90 inhibitor therapies, identifying sensitive patient populations will be essential. Osteopontin (OPN), a secreted integrin-binding glycophosphoprotein, ... More