ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.

製品番号（カタログ番号）	数量
A1049201	100 mL

製品番号（カタログ番号） A1049201

価格（JPY）

4,300

100 mL

ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library.

cGMP manufacturing and quality system

ACK Lysing Buffer is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

研究用途にのみご使用ください。診断目的には使用できません。

化学物質名または材質Lysing Buffer

推奨保存方法Storage conditions: 15–30°C. Protect from light., Shipping conditions: ambient, Shelf life: 12 months from date of manufacture

品質保持期間12 Months

無菌性Sterile

濃度10X

使用対象（アプリケーション）Mammalian Cell Culture

グレードCell Culture

物理的フォームLiquid

製品ラインGibco

数量100 mL

溶液タイプBuffer

pH7 to 7.6

Unit SizeEach

よくあるご質問（FAQ）

What alternative do you offer for ACK Lysing Buffer (Cat. No. A1049201)?

Alternative products we offer are eBioscience 1X RBC Lysis Buffer (Cat. No. 00-4333-57) or eBioscience 10X RBC Lysis Buffer (Multi-species) (Cat. No. 00-4300-54).

Can I use ACK Lysing Buffer with heparinized blood?

Yes, ACK Lysing Buffer can be used with heparinized blood. The principle of lysis is osmotic treatment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I use ACK Lysing Buffer to lyse red blood cells?

A volume of ACK lysing buffer corresponding to 10-20 times the volume of the blood sample is sufficient to lyse red blood cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (1)

引用および参考文献

Abstract

Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.

Authors:Avci FY, Li X, Tsuji M, Kasper DL,

Journal:Nat Protoc

PubMed ID:23196974

Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More