Are the vials of ArC Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD Fixable dead cell stain kits) (Cat. No. A10346, A10648) compatible with Vaporized Hydrogen Peroxide (VHP) sterilization?
While the polypropylene cap is compatible with Vaporized Hydrogen Peroxide (VHP) sterilization, we have not tested the compatibility of the Low-Density Polyethylene (LDPE) / Linear Low-Density Polyethylene (LLDPE) vials with VHP sterilization.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What is the stability for the ArC Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD Fixable dead cell stain kits)?
When stored as directed (2-8 degrees C), this kit is stable for at least 1 year. Please do not freeze the kit. Please visit the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/arc_amine_reactive_comp_bead_kit_man.pdf) for further technical details.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
My ArC Amine Reactive Compensation beads are excessively clumped and cannot be resuspended by vortexing or sonication. What may have caused this?
This can occur if the solution of beads was ever frozen. The formation of ice excludes the beads and compresses the beads together. Once frozen, the beads cannot be resuspended into solution and are no longer usable.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
For the ArC Amine Reactive Compensation Bead Kit, why must I use freshly prepared amine-reactive dye?
This is because succinimidyl ester (NHS ester) groups are very labile to water; they dissociate readily from the dye structure if held in solvents that are contaminated with water, as is possible from condensation or absorbing water from the atmosphere.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Besides the Live/Dead Fixable Dead Cell stain, may I conjugate other amine-reactive dyes to the beads in the ArC Amine Reactive Compensation Bead Kit?
Yes. The succinimidyl ester (NHS ester) and isothiocyanate (e.g., FITC, TRITC) dye derivatives may be attached to the ArC beads, but you would have to optimize the amount of reactive dye per ArC beads.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.