Why can Gibco Geltrex Matrix be used for adhesion-related research, as opposed to Gibco AlgiMatrix 3D Culture System which cannot?
Geltrex Matrix is a basement membrane extract that contains collagen IV, laminin, and other matrix proteins and growth factors that are required for cell adhesion. AlgiMatrix Matrix contains only alginate which does not facilitate cell adhesion. There is nothing in the alginate sponges for the cells to interact with.
Can other cell viability reagents be used with AlgiMatrix Matrix?
Other than the Invitrogen alamarBlue Cell Viability Reagent, AlgiMatrix Matrix is also compatible with Invitrogen PrestoBlue Cell Viability Reagent and Invitrogen CyQuant Direct Cell Proliferation Assay. The protocol is almost the same as the 2D cell culture, but it needs the blank AlgiMatrix Matrix as a blank control.
How important is calcium in the AlgiMatrix firming and dissolving buffers?
The firming buffer works by adding calcium to the sponge, making it stiffer. The dissolving buffer works by removing calcium from the sponge, making it softer.
Is there any assay variability when using AlgiMatrix Matrix?
3D cell culture with AlgiMatrix Matrix approaches a more physiological state in terms of cell growth and behavior, but along with that comes the higher variability typically associated with more complex systems. You may need to do more replicates or more controls.