Blasticidin S HCl (10 mg/mL)
Blasticidin S HCl (10 mg/mL)
Citations & References (5)
Gibco™

Blasticidin S HCl (10 mg/mL)

ブラストサイジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。これは細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞に対しても有効です。ブラストサイジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択試薬として使用されます詳細を見る
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製品番号（カタログ番号）数量
A111390220 mL
A111390310 x 1 mL
製品番号（カタログ番号） A1113902
価格（JPY）
96,400
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Ends: 26-Dec-2025
160,700
割引額 64,300 (40%)
Each
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数量:
20 mL
ブラストサイジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。これは細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞に対しても有効です。ブラストサイジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択試薬として使用されます。推奨される使用濃度は、細胞株に応じて1～30 µg/mL、細菌の選択には25–100 µg/mLです。細胞死は急速に起こり、ブラスティチジン耐性の安定した哺乳類細胞株は1週間未満で生成できます。

ブラストサイジンSに対する耐性は、セレウス菌K55-Sおよびアスペルギルステルレウスからそれぞれ分離されたBSRおよびBSDによりもたらされます。BSR耐性遺伝子はブラストサイジンSデアミナーゼをエンコードします。これはブラストサイジンSのdeaminohydroxyblasticidin Sへの変換を触媒します。Deaminohydroxyblasticidin Sは生物学的に不活性なブラストサイジンSの誘導体であり、原核生物と真核生物のいずれのリボソームにも作用または阻害しません。また、BSD耐性遺伝子はブラストサイジンSデアミナーゼをエンコードし、これはBSRデアミナーゼと同様の反応を触媒します。細菌の選択を目的とした場合、LB培地の塩含有量は低く（<90 mM）、pHは7.0を超えないようにして、ブラストサイジンSの活性を維持する必要があります。耐性のない細胞を死滅させるためにもっとも効率の低いブラストサイジンS濃度を決定するために、殺菌曲線を推奨します。

アプリケーション
 哺乳類細胞、大腸菌、酵母のブラストサイジン選択に関するプロトコルの詳細をご覧ください。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプEukaryotic Cells, Prokaryotic Cells
濃度10 mg/mL
培養タイプMammalian Cell Culture, Insect Cell Culture
製品ラインGibco
数量20 mL
品質保持期間9 Months
形状Liquid
製品タイプAntibiotic
無菌性Sterile-filtered
添加剤ありHEPES
Unit SizeEach
組成および保存条件
Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture

よくあるご質問（FAQ）

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl (10 mg/mL), 20 mL FAQs

引用および参考文献 (5)

引用および参考文献
Abstract
A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.
Authors:Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung R, de March C, Bloom JS, Matsunami H, Kosuri S
Journal:Cell Syst
PubMed ID:30904378
'G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to ... More
A Brain Penetrant Mutant IDH1 Inhibitor Provides In Vivo Survival Benefit.
Authors:Kopinja J, Sevilla RS, Levitan D, Dai D, Vanko A, Spooner E, Ware C, Forget R, Hu K, Kral A, Spacciapoli P, Kennan R, Jayaraman L, Pucci V, Perera S, Zhang W, Fischer C, Lam MH
Journal:Sci Rep
PubMed ID:29062039
'Mutations in IDH1 are highly prevalent in human glioma. First line treatment is radiotherapy, which many patients often forego to avoid treatment-associated morbidities. The high prevalence of IDH1 mutations in glioma highlights the need for brain-penetrant IDH1 mutant-selective inhibitors as an alternative therapeutic option. Here, we have explored the utility ... More
Biological Characterization of a Stable Effector Functionless (SEFL) Monoclonal Antibody Scaffold in Vitro.
Authors:Liu L, Jacobsen FW, Everds N, Zhuang Y, Yu YB, Li N, Clark D, Nguyen MP, Fort M, Narayanan P, Kim K, Stevenson R, Narhi L, Gunasekaran K, Bussiere JL
Journal:J Biol Chem
PubMed ID:27994063
The stable effector functionLess (SEFL) antibody was designed as an IgG1 antibody with a constant region that lacks the ability to interact with Fc? receptors. The engineering and stability and pharmacokinetic assessments of the SEFL scaffold is described in the accompanying article (Jacobsen, F. W., Stevenson, R., Li, C., Salimi-Moosavi, ... More
Proteomics reveals Rictor as a noncanonical TGF-ß signaling target during aneurysm progression in Marfan mice.
Authors:Parker SJ, Stotland A, MacFarlane E, Wilson N, Orosco A, Venkatraman V, Madrid K, Gottlieb R, Dietz HC, Van Eyk JE
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:30004239
The objective of the present study was to 1) analyze the ascending aortic proteome within a mouse model of Marfan syndrome (MFS; Fbn1
Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.
Authors:Nakazawa Y, Hara Y, Oka Y, Komine O, van den Heuvel D, Guo C, Daigaku Y, Isono M, He Y, Shimada M, Kato K, Jia N, Hashimoto S, Kotani Y, Miyoshi Y, Tanaka M, Sobue A, Mitsutake N, Suganami T, Masuda A, Ohno K, Nakada S, Mashimo T, Yamanaka K, Luijsterburg MS, Ogi T
Journal:Cell
PubMed ID:32142649
Transcription-coupled nucleotide excision repair (TC-NER) is initiated by the stalling of elongating RNA polymerase II (RNAPIIo) at DNA lesions. The ubiquitination of RNAPIIo in response to DNA damage is an evolutionarily conserved event, but its function in mammals is unknown. Here, we identified a single DNA damage-induced ubiquitination site in ... More