Blasticidin S HCl (10 mg/mL)

Citations & References (7)

Gibco™

Blasticidin S HCl (10 mg/mL)

Name: Blasticidin S HCl (10 mg/mL)
SKU: A1113903

ブラストサイジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。これは細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞に対しても有効です。ブラストサイジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択試薬として使用されます詳細を見る

テクニカルサポートオーダーサポート

表示を変更

製品番号（カタログ番号）	数量
A1113903	10 x 1 mL
A1113902	20 mL

2 オプション

製品番号（カタログ番号） A1113903

価格（JPY）

84,200

Each

お問い合わせください ›

数量:

10 x 1 mL

ブラストサイジンSは、ストレプトミセスグリセオクロモ遺伝子から単離されたヌクレオチド抗生物質です。これは細菌や真核生物におけるタンパク質合成の強力な阻害剤であり、真菌類、線虫、腫瘍細胞に対しても有効です。ブラストサイジンSは、放出因子によって誘発されるペプチジル-tRNAの加水分解を阻害することで作用し、ペプチド結合形成を阻害します。哺乳類細胞と細菌細胞の両方で選択試薬として使用されます。推奨される使用濃度は、細胞株に応じて1～30 µg/mL、細菌の選択には25–100 µg/mLです。細胞死は急速に起こり、ブラスティチジン耐性の安定した哺乳類細胞株は1週間未満で生成できます。

ブラストサイジンSに対する耐性は、セレウス菌K55-Sおよびアスペルギルステルレウスからそれぞれ分離されたBSRおよびBSDによりもたらされます。BSR耐性遺伝子はブラストサイジンSデアミナーゼをエンコードします。これはブラストサイジンSのdeaminohydroxyblasticidin Sへの変換を触媒します。Deaminohydroxyblasticidin Sは生物学的に不活性なブラストサイジンSの誘導体であり、原核生物と真核生物のいずれのリボソームにも作用または阻害しません。また、BSD耐性遺伝子はブラストサイジンSデアミナーゼをエンコードし、これはBSRデアミナーゼと同様の反応を触媒します。細菌の選択を目的とした場合、LB培地の塩含有量は低く（<90 mM）、pHは7.0を超えないようにして、ブラストサイジンSの活性を維持する必要があります。耐性のない細胞を死滅させるためにもっとも効率の低いブラストサイジンS濃度を決定するために、殺菌曲線を推奨します。

アプリケーション
哺乳類細胞、大腸菌、酵母のブラストサイジン選択に関するプロトコルの詳細をご覧ください。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

細胞タイプEukaryotic Cells, Prokaryotic Cells

濃度10 mg/mL

培養タイプMammalian Cell Culture, Insect Cell Culture

製品ラインGibco

数量10 x 1 mL

品質保持期間9 Months

形状Liquid

製品タイプAntibiotic

無菌性Sterile-filtered

添加剤ありHEPES

Unit SizeEach

組成および保存条件

Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture

よくあるご質問（FAQ）

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl (10 mg/mL), 10 x 1 mL FAQs

引用および参考文献 (7)

引用および参考文献

Abstract

Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.

Authors:Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ

Journal:Nature

PubMed ID:30971826

'Functional genomics approaches can overcome limitations-such as the lack of identification of robust targets and poor clinical efficacy-that hamper cancer drug development. Here we performed genome-scale CRISPR-Cas9 screens in 324 human cancer cell lines from 30 cancer types and developed a data-driven framework to prioritize candidates for cancer therapeutics. We integrated cell ... More

ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer.

Authors:Chen W, Schwalie PC, Pankevich EV, Gubelmann C, Raghav SK, Dainese R, Cassano M, Imbeault M, Jang SM, Russeil J, Delessa T, Duc J, Trono D, Wolfrum C, Deplancke B

Journal:Nat Commun

PubMed ID:31000713

'Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revealed that KFZPs contribute to a wide variety of other biological processes. Using murine and human in vitro and in ... More

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA.

Authors:Shu X, Liu M, Lu Z, Zhu C, Meng H, Huang S, Zhang X, Yi C

Journal:Nat Chem Biol

PubMed ID:29785056

'Uracil in DNA can be generated by cytosine deamination or dUMP misincorporation; however, its distribution in the human genome is poorly understood. Here we present a selective labeling and pull-down technology for genome-wide uracil profiling and identify thousands of uracil peaks in three different human cell lines. Surprisingly, uracil is ... More

CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.

Authors:Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA

Journal:Nat Biotechnol

PubMed ID:28369033

Large genome-mapping consortia and thousands of genome-wide association studies have identified non-protein-coding elements in the genome as having a central role in various biological processes. However, decoding the functions of the millions of putative regulatory elements discovered in these studies remains challenging. CRISPR-Cas9-based epigenome editing technologies have enabled precise perturbation ... More

Optogenetic control of kinetochore function.

Authors:Zhang H, Aonbangkhen C, Tarasovetc EV, Ballister ER, Chenoweth DM, Lampson MA

Journal:Nat Chem Biol

PubMed ID:28805800

Kinetochores act as hubs for multiple activities during cell division, including microtubule interactions and spindle checkpoint signaling. Each kinetochore can act autonomously, and activities change rapidly as proteins are recruited to, or removed from, kinetochores. Understanding this dynamic system requires tools that can manipulate kinetochores on biologically relevant temporal and ... More

View all Blasticidin S HCl (10 mg/mL), 10 x 1 mL citations