A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization.
Authors:Cau J, Faure S, Comps M, Delsert C, Morin N
Journal:J Cell Biol
PubMed ID:11733543
'Coordination of the different cytoskeleton networks in the cell is of central importance for morphogenesis, organelle transport, and motility. The Rho family proteins are well characterized for their effects on the actin cytoskeleton, but increasing evidence indicates that they may also control microtubule (MT) dynamics. Here, we demonstrate that a ... More
Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF.
Authors:Macounová K, Cabrera CR, Yager P
Journal:Anal Chem
PubMed ID:11321320
'The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 ... More
iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks.
Authors:Rockel S, Geertz M, Hens K, Deplancke B, Maerkl SJ
Journal:Nucleic Acids Res
PubMed ID:23258699
Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF-DNA interactions, generating and interrogating an array ... More