Hexokinase inhibits flux of fluorescently labeled ATP through mitochondrial outer membrane porin.
Authors:Perevoshchikova IV, Zorov SD, Kotova EA, Zorov DB, Antonenko YN,
Journal:FEBS Lett
PubMed ID:20412805
'Mitochondrial function requires maintaining metabolite fluxes across the mitochondrial outer membrane, which is mediated primarily by the voltage dependent anion channel (VDAC). We applied fluorescence correlation spectroscopy (FCS) to study regulation of the VDAC functional state by monitoring distribution of fluorescently labeled ATP (BODIPY-FL-ATP) in isolated intact rat liver and ... More
Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding.
Authors:Armbrüster A, Hohn C, Hermesdorf A, Schumacher K, Börsch M, Grüber G
Journal:FEBS Lett
PubMed ID:15792803
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit C than the ADP-analogues. Site-directed mutagenesis and N-terminal sequencing of ... More
ATP modulation of excitatory synapses onto interneurons.
Authors:Khakh BS, Gittermann D, Cockayne DA, Jones A
Journal:J Neurosci
PubMed ID:12917379
Inhibitory interneurons play important roles in neuronal circuits, but the synaptic mechanisms that regulate excitatory input onto interneurons remain to be fully understood. We show that ATP-gated presynaptic P2X2 channels facilitate excitatory transmission onto stratum radiatum interneurons but not onto CA1 pyramidal neurons. ATP released endogenously during carbachol-induced oscillations facilitates ... More
Detection of adenylyl cyclase activity using a fluorescent ATP substrate and capillary electrophoresis.
Authors:Cunliffe JM, Sunahara RK, Kennedy RT
Journal:Anal Chem
PubMed ID:16536404
A capillary electrophoresis laser-induced fluorescence (CE-LIF) assay was developed for detection of adenylyl cyclase (AC) activity using BODIPY FL ATP (BATP) as substrate. In the assay, BATP was incubated with AC and the resulting mixture of BATP and enzyme product (BODIPY cyclic AMP, BcAMP) separated in 5 min by CE-LIF. ... More
Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.