Store in refrigerator (2–8°C) and protect from light.
よくあるご質問(FAQ)
Can the Hibernate-E Medium (Cat. No. A1247601) be used for plating and growing mouse primary hippocampal neurons?
Hibernate-E medium (Cat. No. A1247601) is designed for the maintenance of embryonic neural tissue and is suitable for short-term maintenance of cultured rat neurons at 37°C in ambient CO2 (up to 2 days) and long-term storage of viable brain tissue at 2 to 8°C (up to a month).
However, it is not recommended for the initial plating and growing of neurons.
What medium can be used to maintain/manipulate neurons at ambient CO2?
We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:
- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.
These two media have the same formulation and differ only in osmolality.
Long-term hibernation of human fetal striatal tissue does not adversely affect its differentiation in vitro or graft survival: implications for clinical trials in Huntington's disease.
Transplantation of human fetal CNS tissue is a promising therapy for neurodegenerative conditions such as Huntington's disease (HD), but its widespread adoption is limited by restricted tissue availability. One method of overcoming this problem would be to store the tissue in hibernation medium, an approach that we reported previously for ... More