Annexin V Conjugates for Apoptosis Detection
Annexin V Conjugates for Apoptosis Detection
Citations & References (14)
Invitrogen™

Annexin V Conjugates for Apoptosis Detection

アネキシンVスタンドアロンAlexa Fluor、APC、Pacific Blue、PE、FITC、およびフローサイトメトリーを使用したビオチンコンジュゲートでアポトーシスの初期段階を検出します。
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製品番号（カタログ番号）励起／発光フローサイトメーターレーザーラインコンジュゲート
A13204ビオチン-X
A13201495／519488Alexa Fluor 488
A13199494／518488FITC
A13202578／603532、561Alexa Fluor 568
A13203590／617532Alexa Fluor 594
A23202346／442UVAlexa Fluor 350
A23204650／665633～637Alexa Fluor 647
A35108555／565532、561Alexa Fluor 555
A35109679／702633～637Alexa Fluor 680
A35110650/660633～637APC（アロフィコシアニン）
A35111565/578488、532、561PE
A35122410／455405Pacific Blue
製品番号（カタログ番号） A13204
価格（JPY）
99,300
Each
お問い合わせください ›
コンジュゲート:
ビオチン-X
アポトーシス検出用のアネキシンVスタンドアロンコンジュゲートにより、初期の細胞アポトーシスを迅速かつ信頼性の高い方法で検出できます。アネキシンVコンジュゲートは、フローサイトメトリーを用いたアポトーシス細胞と非アポトーシス細胞の蛍光シグナル強度の差を最大100倍にします。
アネキシンVはホスファチジルセリン（PS）に対する高い親和性を有しており、アポトーシスを受けている細胞の外側のリーフレットに曝露されます。この親和性により、蛍光標識アネキシンV試薬はアポトーシス研究で一般的に使用されます。

アネキシンVコンジュゲートは、アポトーシスの中間期指標であるホスファチジルセリンの外在化を研究するための迅速で信頼性の高い検出法を提供します。フローサイトメトリーで測定した当社の蛍光アネキシンVコンジュゲートで染色したアポトーシス細胞と非アポトーシス細胞の蛍光強度の差は、通常約100倍です。

Nexins Research社との提携により、当社はAlexa Fluor 350、488、555、568、594、647および680アネキシンVコンジュゲート、アネキシンV APC、Biotin-X、FITC、Pacific Blue、およびPEコンジュゲートなどの最高レベルのアネキシンVコンジュゲート提供します。蛍光性の高いアネキシンVコンジュゲートは、アポトーシスの初期指標の1つであるホスファチジルセリンの外在化を研究するための迅速で信頼性の高い検出法を提供します。

アネキシンV Pacific Blueコンジュゲートはバイオレット励起性であり、バイオレットレーザーを備えた装置や、緑色または赤色蛍光色素を含むマルチカラー実験に最適です。

当社のアネキシンVコンジュゲートの利点：
•より明るいシグナルを得るためのInvitrogen Alexa FluorおよびeFluor色素に対するコンジュゲート
•利用可能なすべてのレーザー用のコンジュゲート
•独立した試薬または使いやすいキットとして利用可能

アポトーシス細胞を検出するためのアネキシンV染色は、生細胞および組織でのみ可能です。サンプルを染色後に固定する場合は、シグナルの一時的な保持を実現するために特定の条件が必要です。これには、アルコールを含まないアルデヒドベースの固定法の使用、Ca2+を含むバッファーの使用、界面活性剤／界面活性剤（洗剤）の回避などが挙げられます。また、アポトーシスアッセイにおけるホスファチジルセリンとアネキシンVとの結合を促進する濃縮アネキシン結合バッファーもご用意しています。

For Research Use Only. Not for use in diagnostic procedures.
仕様
概要アネキシンV、ビオチン-Xコンジュゲート
使用対象 (装置)フローサイトメーター
キット内容1バイアルのアネキシンV、ビオチン-xコンジュゲートを含みます。
反応数100
製品タイプアネキシンVコンジュゲート
数量500 μL
出荷条件湿氷
コンジュゲートビオチン-X
Unit SizeEach
組成および保存条件
冷蔵庫（2～8℃）に保存。

よくあるご質問（FAQ）

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am trying to label adherent cells with annexin V and am finding that everything is getting labeled. How can I fix this?

Treating cells with trypsin or other reagents to detach adherent cells causes damage to the membrane, such that cells will be labeled with annexin V. The best way to avoid this problem is to allow your cells to recover for 30-45 min in the incubator. Swirl the tube/plate/flask every few minutes to prevent re-attachment. After this recovery period, you can label your cells with annexin V and analyze by flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

引用および参考文献 (14)

引用および参考文献
Abstract
Regulation of the Fas death pathway by FLICE-inhibitory protein in primary human B cells.
Authors:Hennino A, Berard M, Casamayor-Pallejà M, Krammer PH, Defrance T
Journal:J Immunol
PubMed ID:10975811
'The Fas/Fas ligand (L) system plays an important role in the maintenance of peripheral B cell tolerance and the prevention of misguided T cell help. CD40-derived signals are required to induce Fas expression on virgin B cells and to promote their susceptibility to Fas-mediated apoptosis. In the current study, we ... More
Detection of apoptotic cells by annexin V labeling at electron microscopy.
Authors:Pellicciari C, Bottone MG, Biggiogera M
Journal:Eur J Histochem
PubMed ID:9359032
'Annexin V is a Ca(++)-binding protein which is widely used as a marker for apoptotic cells, as it binds to the phosphatidylserine residues exposed at the surface of apoptotic cells. In this paper, we describe a method for the immunogold-labeling of biotin-conjugated Annexin V, to detect apoptotic thymocytes at electron ... More
Spatiotemporal distribution of dying neurons during early mouse development.
Authors:van den Eijnde SM, Lips J, Boshart L, Vermeij-Keers C, Marani E, Reutelingsperger CP, De Zeeuw CI
Journal:Eur J Neurosci
PubMed ID:10051772
'Apoptosis is a critical cellular event during several stages of neuronal development. Recently, we have shown that biotinylated annexin V detects apoptosis in vivo in various cell lineages of a wide range of species by binding to phosphatidylserines that are exposed at the outer leaflet of the plasma membrane. In ... More
In situ detection of apoptosis during embryogenesis with annexin V: from whole mount to ultrastructure.
Authors:van den Eijnde SM, Luijsterburg AJ, Boshart L, De Zeeuw CI, van Dierendonck JH, Reutelingsperger CP, Vermeij-Keers C
Journal:Cytometry
PubMed ID:9415414
'Apoptosis is of paramount importance during embryonic development. This insight stems from early studies which correlated cell death to normal developmental processes and now has been confirmed by linking aberrant cell death patterns to aberrant development. Linking apoptosis to the phenotype of a developing organism requires spatial information on the ... More
Complement-independent, peroxide-induced antibody lysis of platelets in HIV-1-related immune thrombocytopenia.
Authors:Nardi M, Tomlinson S, Greco MA, Karpatkin S
Journal:Cell
PubMed ID:11551503
'Immunologic thrombocytopenia is seen commonly in HIV-1 infection. The pathogenesis of this problem has been unclear, but it is associated with circulating immune complexes that contain platelet membrane components and anti-platelet membrane GPIIIa49-66 IgG antibodies. These antibodies cause acute thrombocytopenia when injected into mice. We now show that purified anti-GPIIIa49-66 ... More
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