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Citations & References (84)
Invitrogen™
ACMA (9-Amino-6-Chloro-2-Methoxyacridine)
ACMA（9-アミノ-6-クロロ-2-メトキシアクリジン）は、選択的にポリ（d（A-T））に結合するDNA干渉物質です。ACMA-DNA複合体の励起（励起／最大発光約419/483詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| A1324 | 100 mg |
製品番号（カタログ番号） A1324
価格（JPY）お問い合わせください ›
58,000
Each
数量:
100 mg
ACMA（9-アミノ-6-クロロ-2-メトキシアクリジン）は、選択的にポリ（d（A-T））に結合するDNA干渉物質です。ACMA-DNA複合体の励起（励起／最大発光約419/483 nm）は、ほとんどのUV光源で可能であり、より短い波長とより長い波長の両方の色素での使用に対応しています。ACMAは、見かけ上は通電状態の膜とも結合し、pH勾配が形成されると消光します。膜全体での陽イオンおよび陰イオンの移動を追跡し、さまざまな膜結合ATPアーゼのプロトンポンプ活性を研究するために広く使用されています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色青色
検出法蛍光
励起波長域419⁄483
使用対象 (装置)蛍光顕微鏡
数量100 mg
出荷条件室温
標識タイプFluorescent Dye
製品タイプ核酸染色
SubCellular Localization核、細胞膜 & 脂質, Lipids, Nucleus
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。
引用および参考文献 (84)
引用および参考文献
Abstract
Saccharomyces cerevisiae lacking Btn1p modulate vacuolar ATPase activity to regulate pH imbalance in the vacuole.
Journal:J Biol Chem
PubMed ID:16423829
'The vacuolar H(+)-ATPase (V-ATPase) along with ion channels and transporters maintains vacuolar pH. V-ATPase ATP hydrolysis is coupled with proton transport and establishes an electrochemical gradient between the cytosol and vacuolar lumen for coupled transport of metabolites. Btn1p, the yeast homolog to human CLN3 that is defective in Batten disease,
Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation.
Journal:J Biol Chem
PubMed ID:11278748
'The 100 kDa a-subunit of the yeast vacuolar (H(+))-ATPase (V-ATPase) is encoded by two genes, VPH1 and STV1. These genes encode unique isoforms of the a-subunit that have previously been shown to reside in different intracellular compartments in yeast. Vph1p localizes to the central vacuole, whereas Stv1p is present in
Subunit D (Vma8p) of the yeast vacuolar H+-ATPase plays a role in coupling of proton transport and ATP hydrolysis.
Journal:J Biol Chem
PubMed ID:10801866
'To investigate the function of subunit D in the vacuolar H(+)-ATPase (V-ATPase) complex, random and site-directed mutagenesis was performed on the VMA8 gene encoding subunit D in yeast. Mutants were selected for the inability to grow at pH 7.5 but the ability to grow at pH 5.5. Mutations leading to
Oligodeoxynucleotides covalently linked to intercalating agents: a new family of gene regulatory substances.
Journal:Biochem Soc Trans
PubMed ID:3709940
Interactions between the F1 and F0 parts in the Escherichia coli ATP synthase. Associations involving the loop region of C subunits.
Journal:J Biol Chem
PubMed ID:9182524
'The N-ethylmaleimide reactivity of c subunits in Escherichia coli F1F0 ATP synthase (ECF1F0) isolated from five mutants, each with a cysteine at a different position in the polar loop region (positions 39, 40, 42, 43, and 44), has been investigated. The maleimide was found to react with Cys placed at