CTS™ KnockOut™ DMEM/F-12

Citations & References (1)

Gibco™

CTS™ KnockOut™ DMEM/F-12

CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

製品番号（カタログ番号）	数量
A1370801	500 mL

製品番号（カタログ番号） A1370801

価格（JPY）

8,600

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500 mL

Gibco CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). It is designed for use with KnockOut SR and KnockOut SR XenoFree CTS supplements.

Consistent results without fetal bovine serum

Designed for consistent performance, CTS KnockOut DMEM/F-12 is part of the KnockOut line of media products that have delivered reliable results for pluripotent stem cell (ESC and iPSC) culture for years. This fetal bovine free medium leads to little or no spontaneous differentiation.

Equivalent morphology

Stem cells cultured in CTS KnockOut DMEM/F-12 media show normal morphology and karyotype when compared to control stem cells. You can also expect equivalent morphology to manually passaged hESCs, even after enzymatic passaging.

Simplified transition into clinical research

The CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications. Gibco CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files (DMF).

細胞ベース、遺伝子ベース、または組織ベースの製品の研究での用途または製造。注意：ヒトまたは動物への直接投与は意図されていません。

細胞タイプEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

使用対象（アプリケーション）Cell and Gene Therapy Research, Development, and Manufacturing

フォーマットBottle

製造品質ISO 13485, MDSAP, FDA-registered, 21 CFR 820

製品タイプCell Culture Medium

数量500 mL

品質保持期間18 months from date of manufacture

出荷条件Ambient

分類Animal Origin-free

形状Liquid

無菌性Sterile-filtered

Unit SizeEach

組成および保存条件

Store at 2–8°C and protect from light.

よくあるご質問（FAQ）

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CTS™ KnockOut™ DMEM/F-12 FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.

Authors:Wiley LA, Burnight ER, DeLuca AP, Anfinson KR, Cranston CM, Kaalberg EE, Penticoff JA, Affatigato LM, Mullins RF, Stone EM, Tucker BA,

Journal:Sci Rep

PubMed ID:27471043

'Immunologically-matched, induced pluripotent stem cell (iPSC)-derived photoreceptor precursor cells have the potential to restore vision to patients with retinal degenerative diseases like retinitis pigmentosa. The purpose of this study was to develop clinically-compatible methods for manufacturing photoreceptor precursor cells from adult skin in a non-profit cGMP environment. Biopsies were obtained ... More