BG-11 Media, optimized for cyanobacteria

Name: BG-11 Media, optimized for cyanobacteria
SKU: A1379901

Gibco™ BG-11培地は、Synechococcus elongatesなどの特定のシアノバクテリアの増殖と維持に最適化されています。製剤はすぐに使用可能な等倍濃度で提供されており、面倒な培地調製ステップを省略できます。各Gibco™ BG-11培地は、受賞歴のあるGibco™ボトルで提供されます。これにより詳細を見る

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製品番号（カタログ番号）	数量
A1379901	1 L
A1379902	6 L

2 オプション

製品番号（カタログ番号） A1379901

価格（JPY）

12,800

Each

お問い合わせください ›

数量:

1 L

Gibco™ BG-11培地は、Synechococcus elongatesなどの特定のシアノバクテリアの増殖と維持に最適化されています。製剤はすぐに使用可能な等倍濃度で提供されており、面倒な培地調製ステップを省略できます。

各Gibco™ BG-11培地は、受賞歴のあるGibco™ボトルで提供されます。これにより、バイオセーフティキャビネットで容易に使用可能となり、汚染のリスクを最小限に抑え、細胞培養の一貫性を向上できます。Cyanobacteria培養において優れたパッケージングと品質、高い信頼性、一貫性の向上により、全体的な効率がアップし、より堅牢なデータが得られます（図1、2）。

研究用途にのみご使用ください。ヒトまたは動物の治療もしくは診断用には使用できません。

仕様

培地タイプ藻類培地

調製法None (Ready-to-Use)

数量1 L

出荷条件室温

形状液体

製品タイプ培地

対象微生物クラスCyanobacteria

Unit SizeEach

組成および保存条件

Gibco™ BG-11増殖培地には以下が含まれます：

•1 LのGibco™ BG-11培地 — 4℃で保存

よくあるご質問（FAQ）

I'm getting low basal expression of my gene of interest when using the pSyn_1 vector. What do you suggest I try?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter for pSyn_6, on the other hand, is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving the high level expression of your gene of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the difference between pSyn_1 and pSyn_6?

The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).

The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the shelf-life/competency of Synechococcus?

After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the mechanism of integration when using the Synechococcus expression kits?

A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the strain of Synechococcus used in your kits?

We no longer offer any strains. Our reagents and protocols were developed using Synechococcus elongatus strain PCC 7942, a model cyanobacterium.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all BG-11 Media, optimized for cyanobacteria FAQs