Essential 8™ Medium
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Essential 8™ Medium
Citations & References (5)
Gibco™

Essential 8™ Medium

Essential 8™培地は、ヒト多能性幹細胞（PSC）の成長および増殖用に特別に調製された、ゼノフリーおよびフィーダーフリーの培地です。当初、James Thomsonの研究室でGuokai Chenらが開発し詳細を見る
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製品番号（カタログ番号）数量
A1517001500 mL
製品番号（カタログ番号） A1517001
価格（JPY）
36,300
Each
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数量:
500 mL
Essential 8™培地は、ヒト多能性幹細胞（PSC）の成長および増殖用に特別に調製された、ゼノフリーおよびフィーダーフリーの培地です。当初、James Thomsonの研究室でGuokai Chenらが開発し（「E8」として発表）、Cellular Dynamics Internationalにより検証されたEssential 8™培地は、広範囲に試験されており、複数のiPSCラインで多能性を維持することが証明されています。さらに、Essential 8™培地はiPSCの生産をスケールアップするために使用されており、核型異常の兆候を示すことなく、50を超える継代のiPSC増殖をサポートするとともに、3つの生殖系列すべてに分化するiPSCの能力を維持することが示されています。

Essential 8™培地の特長：

•一貫性 — 既存のフィーダーフリー培養培地と比較して、変動が少ない
• 堅牢 — ゼノフリー、cGMP、8成分培地を使用した信頼性の高い堅牢な培養
• 経済的 — 他のフィーダーフリー培地と比較して経済的でスケーラブルなPSC培養

変動の減少
Essential 8™培地はゼノフリーであり、幹細胞培養に必要な8つの必須成分のみが含まれています。20を超える非常に変動性の高い成分を含む他の培地とは異なり、Essential 8™培地はcGMPで製造され、最適化された製剤と増殖因子レベルを備えているため、変動を最小限に抑えながら、細胞の健康、多能性、成長を最大限に高めることができます。

信頼性の高い堅牢な培養
Essential 8™培地は、多能性幹細胞の増殖をサポートし、他のフィーダーシステムと比較して優れた形態と増殖動態を培養に提供することが示されています。

経済的
Essential 8™培地は便利な2成分キット（500 ml基本培地および10 mlサプリメント）で提供され、ビトロネクチン（VTN-N）と併用すると、ヒト多能性幹細胞（PSC）のフィーダーフリー培養のためのコスト効率の高い定義済みシステムを提供します。

Essential 8™培地は、Cellular Dynamics Internationalと提携して商品化されています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞株Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
細胞タイプEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
濃度Essential 8 Basal Medium (1X), Essential 8 Supplement (50X)
製造品質cGMP for medical devices, 21 CFR Part 820 and ISO 13485
製品ラインEssential 8
製品タイプStem Cell Media
数量500 mL
品質保持期間12 Months
出荷条件Supplement - dry ice, Basal - ambient
種Human
分類Xeno-free
Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
形状Liquid
Serum LevelSerum-free
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありPhenol Red
Unit SizeEach
組成および保存条件
• 500 mL basal medium (store at 2—8°C and protect from light)
• 10 mL supplement (store at -5 to -20°C and protect from light)

よくあるご質問（FAQ）

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the glucose concentration in Essential 8 Medium (Cat. No. A1517001)?

Essential 8 Basal Medium contains 3151 mg/L of glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the pH range for Essential 8 Medium?

The pH range for Essential 8 Medium is pH 7 -7.4.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for Essential 8 Medium?

We do provide osmolality information on the certificate of analysis for the particular lot number of your medium. All lots of Essential 8 Medium will meet the osmolality specification of ≥290 to ≤340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I filter sterilize Essential 8 Medium using a 0.22 µm filter?

You can filter the basal Essential 8 medium or the complete Essential 8 medium through a 0.22 µm filter (vacuum driving filtration). But, we recommend that you use a low protein-binding filter.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Essential 8™ Medium FAQs

引用および参考文献 (5)

引用および参考文献
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
Authors:Monteil V, Kwon H, Prado P, Hagelkrüys A, Wimmer RA, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero JP, Wirnsberger G, Zhang H, Slutsky AS, Conder R, Montserrat N, Mirazimi A, Penninger JM
Journal:Cell
PubMed ID:32333836
'We have previously provided the first genetic evidence that angiotensin converting enzyme 2 (ACE2) is the critical receptor for severe acute respiratory syndrome coronavirus (SARS-CoV), and ACE2 protects the lung from injury, providing a molecular explanation for the severe lung failure and death due to SARS-CoV infections. ACE2 has now ... More
Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.
Authors:Reimer A, Vasilevich A, Hulshof F, Viswanathan P, van Blitterswijk CA, de Boer J, Watt FM,
Journal:Sci Rep
PubMed ID:26757610
It is well established that topographical features modulate cell behaviour, including cell morphology, proliferation and differentiation. To define the effects of topography on human induced pluripotent stem cells (iPSC), we plated cells on a topographical library containing over 1000 different features in medium lacking animal products (xeno-free). Using high content ... More
Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems.
Authors:Badenes SM, Fernandes TG, Cordeiro CS, Boucher S, Kuninger D, Vemuri MC, Diogo MM, Cabral JM,
Journal:PLoS One
PubMed ID:26999816
Human induced pluripotent stem (hiPS) cell culture using Essential 8™ xeno-free medium and the defined xeno-free matrix vitronectin was successfully implemented under adherent conditions. This matrix was able to support hiPS cell expansion either in coated plates or on polystyrene-coated microcarriers, while maintaining hiPS cell functionality and pluripotency. Importantly, scale-up ... More
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Authors:Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, Zhang T, Kim TW, Harschnitz O, Redmond D, Houghton S, Liu C, Naji A, Ciceri G, Guttikonda S, Bram Y, Nguyen DT, Cioffi M, Chandar V, Hoagland DA, Huang Y, Xiang J, Wang H, Lyden D, Borczuk A, Chen HJ, Studer L, Pan FC, Ho DD, tenOever BR, Evans T, Schwartz RE, Chen S
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from ... More