Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix
DISCONTINUATION NOTICE: Geltrex™ Stem Cell Qualified Matrices won't be restocked — switch to Geltrex™ Flex Stem Cell Qualified Matrices with equivalent performance, expanded vial sizes, and a NEW organoid-qualified option.
Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix
Citations & References (3)
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Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Geltrex hESC適格な、すぐに使用可能な還元増殖因子基底膜マトリックスは、マウスエンゲルブレスホルムスウォーム（EHS）腫瘍から抽出された基底膜の可溶性形態です。この希釈済みのマトリックスは、冷蔵庫から直接取り出して細胞培養プレートに分注することができ、それ以上の操作は必要ありません。Geltrex詳細を見る
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製品番号（カタログ番号）数量
A156960150 mL
NOTICE: This product will no longer be restocked — switch now to Geltrex™ Flex hESC Qualified matrix, delivering the same trusted performance in expanded vial sizes, along with a new organoid qualified matrix choice.
製品番号（カタログ番号） A1569601
現在の製品を選択する
Geltrex hESC適格な、すぐに使用可能な還元増殖因子基底膜マトリックスは、マウスエンゲルブレスホルムスウォーム（EHS）腫瘍から抽出された基底膜の可溶性形態です。この希釈済みのマトリックスは、冷蔵庫から直接取り出して細胞培養プレートに分注することができ、それ以上の操作は必要ありません。Geltrex hESC適格な、すぐに使用可能な基底膜マトリックスは、StemPro hESC SFMを使用した幹細胞培養アプリケーション用に最適化されています。StemFlex培地または精油8培地システムを使用した幹細胞培養には、Geltrex LDEVフリー、hESC適格な還元増殖因子基底膜マトリックスを使用します。

Geltrex hESC適格なすぐに使用可能なマトリックス：

•一貫—1：100の濃度で希釈済みであるため、確実に標準細胞培養プレートへの幹細胞の付着に最適なタンパク質濃度になります
•安定—4℃で保管すると、一晩ではドライアイス上で融解しません
•経済的—すぐに使用可能なフォーマットであるため、複数ステップの調製や製造失敗の可能性がありません

ばらつき軽減
Geltrex hESC適格なすぐに使用可能なマトリックスは、1：100希釈の緩衝液として提供されていて、コーティング用に細胞培養プレートに直接添加できます。各ロットは、最適な幹細胞接着のために適切なタンパク質濃度で調製されているため、マトリックスのすべてのロットの希釈スキームを計算する必要が最小限に抑えられます。

高信頼性
このマトリックスは、製品の寿命を満たすために4℃で保管するように設計されていて、細胞培養培地と共に冷蔵庫で保管できます—一晩で氷の槽に浸かってしまうような失敗がなく、ゲル化してしまうリスクもありません。さらに、すべてのロットはヒト胚性幹細胞を使用して機能的に認可されており、ラクトース脱水素酵素上昇ウイルス（LDEV）がないことを確認するために試験されているため、すべての細胞培養ラボで安全に使用できます。

優れた費用対効果
Geltrex hESC適格なすぐに使用可能なマトリックスでは複数のステップ（—凍結、解凍、希釈、分注）を行う必要はありません。この製品は本当に時間を節約します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプStem Cells (Hematopoietic, Neural, Mesenchymal, Mouse, Embryonic, iPS - Induced Pluripotent Stem, Human)
濃度0.12 to 0.18 mg/mL
エンドトキシンレベルLow
フォーマットBottle(s)
製品ラインGeltrex
製品タイプBasement Membrane Matrix
純度または品質グレードES Cell-Qualified
数量50 mL
品質保持期間18 Months
種Mouse
分類Reduced Growth Factor
Culture TypeAdherent Cell Culture
形状Liquid
Serum LevelSerum-free
無菌性Sterile
添加剤なしNo Phenol Red
Unit SizeEach
組成および保存条件
Store in refrigerator (2–8°C) and protect from light.

よくあるご質問（FAQ）

Can I use the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) with other PSC media systems (i.e. StemFlex, Essential 8, Essential 8 Flex, etc.)?

No, the Ready-To-Use Geltrex matrix was developed for use with the StemPro hESC SFM System. It is not recommended for other PSC media systems.

For other PSC media systems, we recommend the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement?

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) is a pre-diluted Geltrex product ready to use for coating culture vessels. There is no need to dilute the solution further. The product has also been QC tested using hESCs to ensure that every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302) is a "concentrated" version of the Geltrex hESC-Qualified, Ready-To-Use above. You can pre-dilute this concentrated solution to pre-coat the culture vessel to grow hESCs at your desired usage concentration. This Geltrex matrix has also been QC tested using hESCs to ensure every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202) is the same as the product above. However, it has not been tested to verify it will support hESC growth and expansion. This is a "general" version and not specific to a cell type or line. A full product description can be found here:

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the Gentamicin sulfate concentration in Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)?

The Gentamycin sulfate concentration in Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) is 10 µg/mL.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What dilution should I use for Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)?

For stem cells, a 1:100 dilution is the recommended starting dilution for Geltrex hESC, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601). Other (lower) dilutions may be necessary for other primary cell types and should be determined empirically.

Do you have a protocol for coating 96-well plates with Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix?

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is in principle a diluted version of the frozen Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix.

Please take a look at page 2 under section “Recover frozen PSCs in complete StemFlex Medium” in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016431_StemFlexMediumKit_UG.pdf) for the volume of Geltrex to use to cover a 6-well, 12-well, or 24-well plate.

Basically,
One well of a 24-well plate has a surface area of 2 cm2 and you need 300 µL of diluted Geltrex per well.
One well of a 48-well plate has a surface area of 1 cm2 and you need 150 µL of diluted Geltrex per well.
One well of a 96-well plate has a surface area of 0.32 cm2 and you need 48 µL (˜50 µL) of diluted Geltrex per well.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules.
Authors:Asumda FZ, Hatzistergos KE, Dykxhoorn DM, Jakubski S, Edwards J, Thomas E, Schiff ER
Journal:
PubMed ID:29626713
'A variety of approaches have been developed for the derivation of hepatocyte-like cells from pluripotent stem cells. Currently, most of these strategies employ step-wise differentiation approaches with recombinant growth-factors or small-molecule analogs to recapitulate developmental signaling pathways. Here, we tested the efficacy of a small-molecule based differentiation protocol for the ... More
The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM).
Authors:Amend N, Thiermann H, Worek F, Wille T
Journal:Toxicol Lett
PubMed ID:30858091
The global use of organophosphorus compounds (OP) for pest control and nerve agents being used in military conflicts and for assassinations renders intoxications by these agents a public health concern. OP-poisoned patients often suffer from dysrhythmias which may ultimately result in death. In this study, human-induced pluripotent stem cells derived ... More
Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells.
Authors:Kitajima R, Nakai R, Imamura T, Kameda T, Kozuka D, Hirai H, Ito H, Imai H, Imamura M
Journal:Stem Cell Res
PubMed ID:32151953
Evolutionary developmental biology of our closest living relative, the chimpanzee (Pan troglodytes), is essential for understanding the origin of human traits. However, it is difficult to access developmental events in the chimpanzee in vivo because of technical and ethical restrictions. Induced pluripotent stem cells (iPSCs) offer an alternative in vitro ... More