7-Amino-4-Methylcoumarin, reference standard
7-Amino-4-Methylcoumarin, reference standard
Citations & References (11)
Invitrogen™

7-Amino-4-Methylcoumarin, reference standard

7-アミノ-4-メチルクマリン（AMC）は、AMCベースのカスパーゼ基質を含むAMCベースの酵素基質の蛍光参照標準として使用できます。AMCの蛍光スペクトルは、生理学的pHの近くまたはそれ以上でアッセイした場合、pH依存性のプロトン化/脱プロトン化による変動の影響を受けません。詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
A191100 mg
製品番号（カタログ番号） A191
価格（JPY）
17,100
Online offer
Ends: 26-Dec-2025
28,600
割引額 11,500 (40%)
Each
お問い合わせください ›
数量:
100 mg
7-アミノ-4-メチルクマリン（AMC）は、AMCベースのカスパーゼ基質を含むAMCベースの酵素基質の蛍光参照標準として使用できます。AMCの蛍光スペクトルは、生理学的pHの近くまたはそれ以上でアッセイした場合、pH依存性のプロトン化/脱プロトン化による変動の影響を受けません。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
発光430 nm
励起351 nm
標識または色素メチルクマリン
分子量175.2
製品タイプ7-アミノ-4-メチルクマリン
数量100 mg
出荷条件室温
標識タイプクマリン色素
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問（FAQ）

What solvent should I use to reconstitute 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191)? Once reconstituted, can the product be aliquoted and stored at -20 degrees C without damaging the molecule?

The 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191) can be resuspended in DMF or DMSO at a concentration of at least 1 mM. The molecular weight is 175.19 g/mol.
Yes, the solution can be stored in aliquots at -20 degrees C for at least 6 months.

Find additional tips, troubleshooting help, and resources within our Protein Standards and Ladders Support Center.

引用および参考文献 (11)

引用および参考文献
Abstract
High-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography of glycosphingolipids.
Authors:Tomono Y, Abe K, Watanabe K
Journal:Anal Biochem
PubMed ID:2327579
'A method combining high-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography is introduced for determination of glycosphingolipids. Glycolipids in crude extract from rat liver were separated quantitatively from neutral lipids and phospholipids with a phenylboronic acid-derivatized silica gel column. Glycolipids were eluted quantitatively with approximately 98% ... More
Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.
Authors:Irvine GB, Ennis M, Williams CH
Journal:Anal Biochem
PubMed ID:2339787
'A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was ... More
New fluorogenic substrates for subtilisin.
Authors:Kanaoka Y, Takahashi T, Nakayama H, Tanizawa K
Journal:Chem Pharm Bull (Tokyo)
PubMed ID:3899387
A new fluorescent tag for labeling of saccharides.
Authors:Prakash C, Vijay IK
Journal:Anal Biochem
PubMed ID:6846798
Potential aldehyde groups of N-acetylglucosamine and its di- and tri-saccharides were coupled with 7-amino-4-methylcoumarin (AMC) by reductive amination in the presence of sodium cyanoborohydride. The products were purified by silica gel chromatography. Acid hydrolysis and digestion with glycosidases gave the expected products. One picomole of the sugar-AMC complexes could be ... More
A new fluorogenic substrate for plasmin.
Authors:Pierzchala PA, Dorn CP, Zimmerman M
Journal:Biochem J
PubMed ID:161507
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of ... More
View all 7-Amino-4-Methylcoumarin, reference standard citations