Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

Citations & References (13)

Invitrogen™

Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

Amplex Red Xanthine/Xanthine Oxidase Assay Kitでは、100 µLのアッセイ容量で精製システム内のわずか200 nMのキサンチンまたはヒポキサンチン、あるいはわずか0.1詳細を見る

製品番号（カタログ番号）	数量
A22182	400 Assays

製品番号（カタログ番号） A22182

価格（JPY）

87,600

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400 Assays

Amplex Red Xanthine/Xanthine Oxidase Assay Kitでは、100 µLのアッセイ容量で精製システム内のわずか200 nMのキサンチンまたはヒポキサンチン、あるいはわずか0.1 mU/mLのキサンチン酸化酵素活性を検出する高感度でシンプルな蛍光測定を行えます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプその他の標識または色素

フォーマットチューブ、96ウェルプレート

数量400 Assays

出荷条件室温

使用対象（アプリケーション）キサンチン／キサンチンオキシダーゼアッセイ

使用対象 (装置)マイクロプレートリーダー, 分光光度計, フルオロメータ

製品ラインAmplex

製品タイプRed Xanthine/Xanthine Oxidase Assay Kit

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (13)

引用および参考文献

Abstract

The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.

Authors:Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C,

Journal:J Endocrinol

PubMed ID:18562629

Endogenous purines including inosine have been shown to exert immunomodulatory and anti-inflammatory effects in a variety of disease models. The dosage of inosine required for protection is very high because of the rapid metabolism of inosine in vivo. The aim of this study was to determine whether a metabolic-resistant purine ... More

Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction.

Authors:Whidden MA, McClung JM, Falk DJ, Hudson MB, Smuder AJ, Nelson WB, Powers SK,

Journal:J Appl Physiol (1985)

PubMed ID:18974366

'Respiratory muscle weakness resulting from both diaphragmatic contractile dysfunction and atrophy has been hypothesized to contribute to the weaning difficulties associated with prolonged mechanical ventilation (MV). While it is clear that oxidative injury contributes to MV-induced diaphragmatic weakness, the source(s) of oxidants in the diaphragm during MV remain unknown. These ... More

Voluntary wheel running restores endothelial function in conduit arteries of old mice: direct evidence for reduced oxidative stress, increased superoxide dismutase activity and down-regulation of NADPH oxidase.

Authors:Durrant JR, Seals DR, Connell ML, Russell MJ, Lawson BR, Folian BJ, Donato AJ, Lesniewski LA,

Journal:J Physiol

PubMed ID:19417091

'Habitual aerobic exercise is associated with enhanced endothelium-dependent dilatation (EDD) in older humans, possibly by increasing nitric oxide bioavailability and reducing oxidative stress. However, the mechanisms involved are incompletely understood. EDD was measured in young (6-8 months) and old (29-32 months) cage control and voluntary wheel running (VR) B6D2F1 mice. ... More

Oxidizing Effects of Exogenous Stressors in Huntington's Disease Knock-in Striatal Cells--Protective Effect of Cystamine and Creatine.

Authors:Ribeiro M, Silva AC, Rodrigues J, Naia L, Rego AC,

Journal:

PubMed ID:24008831

'Huntington''s disease (HD) is a polyglutamine-expansion disease associated to degeneration of striatal and cortical neurons. Previously, we showed that oxidative stress occurs in HD knock-in striatal cells, but little is known regarding cell antioxidant response against exogenous stimuli. Therefore, in the present study we analyzed cellular antioxidant profile following hydrogen ... More

Preserved immune functions and controlled leukocyte oxidative stress in naturally long-lived mice: possible role of nuclear factor kappa B.

Authors:Arranz L, Caamaño JH, Lord JM, De la Fuente M,

Journal:J Gerontol A Biol Sci Med Sci

PubMed ID:20558495

'In order to verify the survival biomarker role of several immune functions, and to determine the oxidation and inflammation mechanisms underlying variability in the aging process, we have investigated a variety of immune functions and oxidative stress parameters as well as activation of the nuclear factor kappa B (NF kappaB) ... More

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