How can I view DNA on my E-Gel agarose gel with SYBR Safe DNA stain?
SYBR Safe-stained gels can be visualized with a standard UV transilluminator, a laser-based scanner equipped with an excitation source in the UV range or between 470 and 530 nm, or a blue-light transilluminator.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
My E-Gel agarose gel has speckles when viewed in the imager. What should I do?
Here are some suggestions:
- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
My sample is leaking from the wells when running my E-Gel agarose gels. What happened?
Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
I accidentally stored my E-Gel agarose gels at 4 degrees C instead of room temperature. Can I still use them?
While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.
Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.
What loading buffer should I use for my E-Gel agarose gels?
Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:
E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA
Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
