Does the PowerUp SYBR Green Master Mix (Cat. No. A25741, A25742, A25743, A25776, A25777, A25778, A25779, A25780, A25919) contain uracil-DNA glycolase?
This master mix has a heat-labile UDG. It is completely and irreversibly inactivated after 10 minute incubation at 50°C.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
What type of samples is the MagMAX DNA Multi-Sample Ultra Kit (Cat. No. A25597) optimized for?
We have optimized protocols for extracting DNA from buccal swabs, whole blood, blood cards (Whatman FTA), oral rinse, saliva, and urine samples. In addition, the kit can be used to process vaginal microbiota samples (e.g., ThinPrep, Surepath) for gDNA extraction from yeast, bacteria (Gram positive/negative), viruses, and protozoa with the addition of additional enzymatic steps.
In the MagMAX DNA Multi-Sample Ultra Kit protocol, why do blood samples appear cloudy after adding the Multi-Sample DNA Lysis Buffer?
On rare occasions, the sample may turn cloudy after adding the Multi-Sample DNA Lysis Buffer. The sample will clear up when isopropanol is added. This will not have any impact on the yield or quality of the DNA.
Can heparin be used to collect blood samples for the MagMAX DNA Multi-Sample Ultra Kit?
Heparin is not recommended as an anti-coagulant since it can cause inhibition of PCR reactions. Instead, we strongly recommend collecting blood samples in EDTA or sodium citrate.
What is the best way to remove the buccal swabs from the MagMAX DNA Multi-Sample Ultra Kit lysate?
We recommend transferring the lysate to a new MagMAX Express-96 Deep Well Plate (Cat. No. 4388476). This option eliminates contamination risks and saves time. To transfer lysates, set a multi-channel micropipetor to 300 µL and transfer one row at a time. Each well should contain 200-250 µL after transfer.