Essential 8™ Adaptation Kit
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Essential 8™ Adaptation Kit
Gibco™

Essential 8™ Adaptation Kit

Essential 8™適応キットには、フィーダー依存培養からフィーダーフリー培養への移行時に最適な生存率を得るためのEssential 8™培地と組み換えヒトラミニン521(rhLaminin-521)が含まれています。rhLaminin-521は、低分子阻害剤が存在しないストレス条件下で細胞生存を促進することが証明されています。さらに、rhLaminin-521は詳細を見る
製品番号(カタログ番号)数量
A259351 Kit
製品番号(カタログ番号) A25935
価格(JPY)
53,000
Each
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数量:
1 Kit
Essential 8™適応キットには、フィーダー依存培養からフィーダーフリー培養への移行時に最適な生存率を得るためのEssential 8™培地と組み換えヒトラミニン521(rhLaminin-521)が含まれています。rhLaminin-521は、低分子阻害剤が存在しないストレス条件下で細胞生存を促進することが証明されています。さらに、rhLaminin-521は、核型異常の兆候なしに20回以上の継代でPSCの増殖をサポートし、PSCがすべての3つの生殖細胞系統に分化する能力を維持することが示されています。rhLaminin-521は別途購入することもできます。

Essential 8の適応キット:
•堅牢–フィーダー依存培養系とフィーダーフリー培養系の間で、最小限の労力で細胞を移行させることができます
• 生理学的に関連性がある–分化を最小限に抑えて細胞を拡張します
• 拡張可能–遺伝的安定性に影響を与えない単細胞としての継代幹細胞株

簡単な移行
rhLaminin-521は、Essential 8培地と併用すると、フィーダー依存培養系からの移行時に多能性幹細胞の接着と増殖をサポートする強固な培養基質を提供します。

信頼できる拡大
rhLaminin-521は胚盤胞で発現しているため、ヒト幹細胞ニッチを模倣した環境を提供します。

容易なスケールアップ
rhLaminin-521は、ROCK阻害剤の非存在下で、単一細胞として安定した多能性幹細胞の維持を促進します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプPluripotent Stem Cells
製品ラインEssential 8
製品タイプStem Cell Adaptation Kit
数量1 Kit
出荷条件Supplement and matrix - dry ice, Basal - ambient
分類Xeno-free
Unit SizeEach
組成および保存条件
• 100 μg rhLaminin-521, store at -10 to -30°C, protect from light
• 500 mL basal medium, store at 2-8°C, protect from light
• 10 mL supplement, store at -5 to -20°C, protect from light

よくあるご質問(FAQ)

Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for blood cells?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of CD34+ blood cells. For CD34+ cells, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 39-44). On Day 3, you can utilize rhVTN-N (Cat. No. A14700), Geltrex (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, reprogrammed CD34+ cells should be fed every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am interested in using StemFlex Medium in somatic cell reprogramming. What protocol should I follow for human fibroblasts?

We have evaluated the use of Cytotune-iPS 2.0 Sendai Reprogramming Kit (Cat. No. A16517) for somatic cell reprogramming of both neonatal and adult human dermal fibroblasts. For fibroblasts, follow the instructions provided in the Cytotune 2.0 reprogramming manual for feeder-free reprogramming (pgs. 16-20). On Day 7, you may use rhVTN-N (Cat. No. A14700), Geltrex matrix (Cat. No. A1413302), or rhLaminin-521 (Cat. No. A29248 or A29249). From Day 8 onward, rather than feeding daily with Essential 8 Medium, we recommend that you feed reprogrammed fibroblasts every-other-day with StemFlex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use Geltrex matrix instead of rhLaminin-521 for clonal expansion workflows where cells are seeded at 1-5 cells per well of a 96-well plate?

We have not yet evaluated the Geltrex matrix system for clonal expansion in the presence of StemFlex Medium. However, rhLaminin-521 does provide optimal survival of cells following single-cell passaging and thus this matrix is recommended for such critical applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What protocol is recommended for delivery of Cas9 protein:guide RNA complex to my PSCs cultured in StemFlex Medium?

We recommend use of the Neon Electroporation device for electroporation of PSCs with Cas9 protein:guide RNA complex following the protocol guidance in the following demonstrated protocol (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1584-PJT1313-COL31227-Demonstrated-Protocol-Performing-CRISPR-Cas9-FHR.pdf); see the section entitled “Knockout by electroporation of RNP using the Neon Transfection System.” We have seen that the Neon electroporation protocols 7 and 14 provide optimal indel formation while maintaining cell survival. However, the electroporation conditions may need to be optimized for your pluripotent cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.