Avidin, NeutrAvidin™, Horseradish Peroxidase conjugate

当社のNeutrAvidin™ビオチン結合タンパク質の西洋ワサビペルオキシダーゼ（HRP）コンジュゲート（糖鎖を除去し、等電点を下げるために処理されたアビジンの一形態）は、非特異的結合によりバックグラウンドを大幅に低下させることができます。アビジンの脱グリコシル化に使用される方法は、その特異的結合を保持します。詳細を見る

製品番号（カタログ番号）	数量
A2664	1 mg

製品番号（カタログ番号） A2664

価格（JPY）

48,500

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当社のNeutrAvidin™ビオチン結合タンパク質の西洋ワサビペルオキシダーゼ（HRP）コンジュゲート（糖鎖を除去し、等電点を下げるために処理されたアビジンの一形態）は、非特異的結合によりバックグラウンドを大幅に低下させることができます。アビジンの脱グリコシル化に使用される方法は、その特異的結合を保持します。

研究用にのみ使用できます。診断用には使用いただけません。

標識または色素HRP(西洋ワサビペルオキシダーゼ)

数量1 mg

出荷条件湿氷

コンジュゲートHRP

製品ラインNeutrAvidin

Unit SizeEach

組成および保存条件

冷凍庫(-5∼-30°C)に保存。

引用および参考文献 (13)

引用および参考文献

Abstract

Suppression of nonspecific binding of avidin-biotin complex (ABC) to proteins electroblotted to nitrocellulose paper.

Authors:Clark RK, Tani Y, Damjanov I

Journal:J Histochem Cytochem

PubMed ID:3772081

'Nitrocellulose blots of cell extracts reacted in sequence with biotinylated lectins and horseradish peroxidase-labeled avidin-biotin complex (ABC) often show considerable nonspecific staining of protein bands. Experiments were performed to determine which of the components of the ABC were responsible for this and whether or not the nature and ionic strength ... More

Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase.

Authors:Leng XH, Nishi T, Forgac M

Journal:J Biol Chem

PubMed ID:10329659

'The membrane topography of the yeast vacuolar proton-translocating ATPase a subunit (Vph1p) has been investigated using cysteine-scanning mutagenesis. A Cys-less form of Vph1p lacking the seven endogenous cysteines was constructed and shown to have 80% of wild type activity. Single cysteine residues were introduced at 13 sites within the Cys-less ... More

Artifactual detection of biotin on histones by streptavidin.

Authors:Bailey LM, Ivanov RA, Wallace JC, Polyak SW,

Journal:Anal Biochem

PubMed ID:17920026

'Biotinylation is a recent addition to the list of reported posttranslational modifications made to histones. Holocarboxylase synthetase (HCS) and biotinidase have been implicated as biotinylating enzymes. However, the details of the mechanism and the regulation of biotin transfer on and off histones remains unclear. Here we report that in a ... More

Stimulation of the pentose phosphate pathway and glutathione levels by dehydroascorbate, the oxidized form of vitamin C.

Authors:Puskas F, Gergely P, Banki K, Perl A

Journal:FASEB J

PubMed ID:10877828

'Ascorbic acid, or vitamin C, generally functions as an antioxidant by directly reacting with reactive oxygen intermediates and has a vital role in defenses against oxidative stress. However, ascorbic acid also has pro-oxidant properties and may cause apoptosis of lymphoid and myeloid cells. The present study shows that dehydroascorbate, the ... More

Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.

Authors:van Roy N, Mangelschots K, Speleman F

Journal:Trends Genet

PubMed ID:8488564

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