The Ion 520 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 520 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times in as little as 2.5 hours for 200 bp sequencing.
The Ion 520 Chip Kit offers:
• Sample tracking functionality from an integrated barcode • A rapid run time of only 2.5–4 hours, generating 3–6 million reads, depending on application • Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing • Easy handling and loading of templated products for sequencing • Compatibility with current library preparation methods
Notes: This kit is only compatible with the Ion S5 and Ion S5 XL systems. Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)Ion GeneStudio S5 System, Ion S5™ XL System
グリーン機能Sustainable disposal
チップあたりの読取り数3-6M
製品タイプChip Kit
数量8 chips
出荷条件Room Temperature
製品ラインIon 520
Unit SizeEach
組成および保存条件
Store at room temperature
よくあるご質問(FAQ)
How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?
The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section Strategies for combining Ion Ampliseq libraries.