The Ion 540™ Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 540 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.
The Ion 540 Chip Kit offers:
• Sample tracking functionality from an integrated barcode • A rapid run time of as little as 2.5 hours for 200 bp sequencing, generating 60–80 million reads • Support for a variety of research applications, including sequencing of up to three human exomes, transcriptome profiling, and targeted resequencing of gene panels • Easy handling and loading of templated products for sequencing • Compatibility with current library preparation methods
Notes: This kit is only compatible with the Ion S5 and Ion S5 XL systems. Use this kit with the Ion 540 Kit-Chef and the Ion 540 Kit-OT2.
仕様
使用対象(アプリケーション)Sequencing
フォーマットKit
グリーン機能Sustainable disposal
チップあたりの読取り数60-80M
製品タイプChip Kit
数量4 Chips
シーケンスタイプGenome and DNA Sequencing
出荷条件Room Temperature
種Human
製品ラインIon 540
Unit SizeEach
組成および保存条件
Store at room temperature
よくあるご質問(FAQ)
Can I use Ion 540 Chip Kit with Ion AmpliSeq Primary Immune Deficiency Research Panel v2? I am worried about the average amplicon length of 243 bp.
Yes, can use Ion 540 chips with an amplicon length of 243 bp. The 200 bp protocol, which is available for all chip sizes including Ion 540 and 550, can be used for amplicons up to 275 bp. Instead, the 400 bp protocol can be used only with Ion 510, 520, and 530 chips with amplicons lengths ranging between 125 and 375 bps.
Can I reuse the Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges for running a second chip separately from the first chip?
The Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges can be reused in a second single-chip templating run if the second run is performed within eight days of the first run.