MagMAX&trade; <i>mir</i>Vana&trade; RNA Isolation Kits
Applied Biosystems™

MagMAX™ mirVana™ RNA Isolation Kits

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types.
製品番号(カタログ番号)製品タイプ数量
A27828Kit96 reactions
A27828B1000Proteinase K960 Reactions
A27828B1001TURBO DNase960 Reactions
A27828B1002Lysis/Binding Enhancer960 Reactions
A27828B1003Proteinase K Digestion Buffer960 Reactions
A27828B1004RNA Binding Beads960 Reactions
A27828B1005TURBO DNase Buffer960 Reactions
A27828B1006Lysis Buffer960 Reactions
A27828B1007Rebinding Buffer960 Reactions
A27828B1008Wash Solution 2 Concentrate960 Reactions
A27828B1009Wash Solution 1 Concentrate960 Reactions
A27828B1010Elution Buffer960 Reactions
A278280Proteinase K96 Reactions
A278281TURBO DNase96 Reactions
A278282Lysis/Binding Enhancer96 Reactions
A278283Proteinase K Digestion Buffer96 Reactions
A278284RNA Binding Beads96 Reactions
A278285TURBO DNase Buffer96 Reactions
A278286Lysis Buffer96 Reactions
A278287Rebinding Buffer96 Reactions
A278288Wash Solution 2 Concentrate96 Reactions
A278289Wash Solution 1 Concentrate96 Reactions
A2782810Elution Buffer96 Reactions
製品番号(カタログ番号) A27828
価格(JPY)
74,500
Each
製品タイプ:
Kit
数量:
96 reactions

Scalable isolation of RNA (including small RNAs)

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:

  • Automation-ready, phenol-free extraction
  • Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
  • Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types

Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering 'specialized' kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

  • Serum/plasma
  • Whole blood
  • Tissue
  • Cell culture
  • Urine

Benefits of MagMAX magnetic bead–based purification

Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

For Research Use Only. Not for use in diagnostic procedures.
仕様
溶出量20 to 100 μL
最終産物タイプトータルRNA
使用対象(アプリケーション)次世代シーケンシング、リアルタイムPCR、microRNA解析
使用対象 (装置)KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
高スループット適合性ハイスループット対応、手動プロトコル、自動プロトコル
製品タイプKit
精製時間Up to 1.5 hr.
数量96 reactions
出荷条件ドライアイス
出発物質量Cells: ≤106, Plasma: ≤100 μL, Serum: ≤100 μL, Tissue: ≤50 mg, Urine: ≤250 μL, Whole Blood: ≤50 μL
収量Cells: ≤32 μg RNA per 50,000 cells, Tissue: ≤500 μg per 5 mg
Isolation TechnologyMagnetic Bead
サンプルタイプ血液, 細胞, 液体サンプル(血清など), RNA, 組織, Whole Blood
TargetmRNA, siRNA, miRNA
Unit SizeEach
組成および保存条件

ボックス1(-15~-25℃で保存)
• プロテイナーゼK(50 mg/mL)1 x 0.48 mL
• 溶解/結合エンハンサー1 x 0.96 mL
• TURBO DNase™(20U/uL)1 x 0.2 mL

ボックス2(室温で保存)
• 溶解バッファー1 x 115 mL
• PK消化バッファー1 x 4.4 mL
• RNA結合ビーズ1 x 2 mL
• 洗浄溶液1濃縮液1 x 20 mL
• 洗浄溶液2濃縮液1 x 60 mL
• 再結合バッファー1 x 4.8 mL
• MagMAX™ TURBO DNase™バッファー1 x 4.6 mL
• 溶出バッファー1 x 9.6 mL
• 処理プレート1枚
• 溶出プレート2枚
• プレートカバー4枚

よくあるご質問(FAQ)

Can I purchase the RNA Binding beads from the MagMAX MirVana Total RNA Isolation Kit (Cat. No. A27828) separately?

Yes, you can. The DNA Binding Beads for MagMAX-96 DNA Multi-Sample Kit (Cat. No. 4489112) are the same as that used in A27828.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the minimum cell number for RNA isolation using MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828)?

We have tested extraction from as low as 1,000 cells using cultured HeLa, Jurkat, and HepG2 cells.

What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.

Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01

What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).