The ProteinSEQ™ Protein A Core Kit is a partial kit that enables users to customize their ProteinSEQ protein measurements for leeched Protein A, or any other ligand used in their purification columns. It contains the buffers and reagents, but not the antibody-loaded capture beads, needed to enable PCR-based quantitation of Protein A or any other purification ligand. The kit contains sufficient reagents for 200 reactions. For a kit that includes Protein A capture beads and standards, for use in the quantitation of Protein A, please see the ProteinSEQ™ Protein A Quantitation Kit (Cat. No. 4469343).
• Quantitate leeched protein contaminants with ultra-high sensitivity • Accelerate process development with more valid test results per run • Reduce assay hands-on time with less sample dilution and preparation • Achieve unprecedented productivity with near-5-log assay dynamic range
ProteinSEQ Protein A quantitation The ProteinSEQ assay platform uses qPCR technology to measure process contaminants with extraordinary sensitivity and unparalleled dynamic range. Protein A or other purification ligands leeching from purification columns can be accurately measured with outstanding spike recovery and dilutional linearity.
Streamlined assay worflow The ProteinSEQ system is the only instrument platform-based Protein A and purification ligand quantitation solution to offer single-digit picogram sensitivity and nearly 5 logs of dynamic range. Laboratory workflow is greatly accelerated due to the reduction in the number of sample dilutions and replicates in each run.
For Research Use Only. Not for use in diagnostic procedures.
仕様
アッセイProtein Quantitation Assay
使用対象(アプリケーション)Proximity Ligation Assay
フォーマットKit
数量200 Reactions
出荷条件Approved for shipment at Room Temperature or on Wet or Dry Ice
製品ラインProteinSEQ
タイプProtein A Core Kit
Unit Size200 reactions
組成および保存条件
Store at 4–8°C: • ProteinSEQ elution buffer • Fast master mix (2X) • Wash buffer • ProteinSEQ diluent
Store at -5 to -30°C: • ProteinSEQ ligation & assay mix (20X) • ProteinSEQ ligase • Protein A sample prep reagent (2X)