Essential 8™ Flex Medium Kit
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Essential 8™ Flex Medium Kit
Citations & References (1)
Gibco™

Essential 8™ Flex Medium Kit

Essential 8™フレックスは、週末のフィードを必要とせずに多能性幹細胞（PSC）の培養をサポートするゼノフリーでフィーダーフリーの培地です。Essential 8フレックスは、成分のばらつきの最小化、ゼノフリーのcGMP生産培地による信頼性が高く堅牢な培養などに加え、柔軟性といったEssential詳細を見る
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製品番号（カタログ番号）数量
A28585011 Kit
製品番号（カタログ番号） A2858501
価格（JPY）
44,800
Each
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数量:
1 Kit
Essential 8™フレックスは、週末のフィードを必要とせずに多能性幹細胞（PSC）の培養をサポートするゼノフリーでフィーダーフリーの培地です。Essential 8フレックスは、成分のばらつきの最小化、ゼノフリーのcGMP生産培地による信頼性が高く堅牢な培養などに加え、柔軟性といったEssential 8培地のすべての利点を提供します標準的なPSCのメンテナンスは、ラボで週7日行う必要がなくなりました。

柔軟性
Essential 8フレックス培地を使用すると、造血幹細胞は分割日と翌日にのみフィードする必要があります。週末など、細胞を48時間以上フィードせずに放置する場合は、金曜日のフィード量を単純に2倍にしてください。

信頼性の高い堅牢な培養
Essential 8フレックス培地は、核型異常の兆候なしに30回以上の継代でPSCの成長をサポートし、iPSCが3つの生殖細胞系列すべてに分化する能力を維持することが示されています。

高い費用対効果
Essential 8フレックス培地は、便利な2成分キット（500mLの基本培地と10mLのサプリメント）で提供され、ビトロネクチン（VTN-N）と一緒に使用すると、ヒトPSCのフィーダーフリー培養のための費用対効果の高い定義されたシステムを提供します。

Essential 8フレックス培地の組成は、ChenらによってEssential 8培地のために発表されたものに基づいており、幹細胞の健康に悪影響を与えることなく、PSC培養液のコントロールと柔軟性を可能にします。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞株Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
細胞タイプEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
濃度Essential 8 Flex Basal Medium (1X), Essential 8 Flex Supplement (50X)
製造品質cGMP for medical devices, 21 CFR Part 820 and ISO 13485
製品ラインEssential 8
製品タイプStem Cell Media
数量1 Kit
品質保持期間12 Months
出荷条件Supplement - dry ice, Basal - ambient
分類Xeno-free
Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
形状Liquid
Serum LevelSerum-free
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありPhenol Red
Unit SizeEach
組成および保存条件
• 500 mL Basal Medium (store at 2–8°C and protect from light)
• 10 mL Supplement (store at -5 to -20°C and protect from light)

よくあるご質問（FAQ）

What is the pH range for Essential 8 Medium?

The pH range for Essential 8 Medium is pH 7 -7.4.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for Essential 8 Medium?

We do provide osmolality information on the certificate of analysis for the particular lot number of your medium. All lots of Essential 8 Medium will meet the osmolality specification of ≥290 to ≤340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I filter sterilize Essential 8 Medium using a 0.22 µm filter?

You can filter the basal Essential 8 medium or the complete Essential 8 medium through a 0.22 µm filter (vacuum driving filtration). But, we recommend that you use a low protein-binding filter.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between StemFlex Medium and Essential 8 Flex Medium?

Essential 8 Flex Medium is a serum-free, xeno-free medium that contains proprietary material to extend the activity of the key heat-sensitive component, FGF2. Therefore, it supports the culture and expansion of feeder-free pluripotent stem cells (PSCs) without the need for daily or weekend feeding. Note: We also offer Essential 8 Medium that does not allow skipping of PSC feeding during the weekend.

StemFlex Medium also supports the expansion of feeder-free PSCs with a flexible feeding schedule (including weekend-free feeding) and also the ability to choose the matrix and passaging reagent that best suits specific applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it possible to reprogram somatic cells using Essential 7 or Essential 8 medium and transition them into StemFlex Medium upon harvest?

Yes. If improved reprogramming efficiency is required, then you may utilize Essential 7 (Essential 6 + bFGF) or Essential 8 Medium for reprogramming somatic cells and directly transition them into StemFlex Medium system upon colony selection.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Essential 8™ Flex Medium Kit FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease.
Authors:Giacomelli E, Meraviglia V, Campostrini G, Cochrane A, Cao X, van Helden RWJ, Krotenberg Garcia A, Mircea M, Kostidis S, Davis RP, van Meer BJ, Jost CR, Koster AJ, Mei H, Míguez DG, Mulder AA, Ledesma-Terrón M, Pompilio G, Sala L, Salvatori DCF, Slieker RC, Sommariva E, de Vries AAF, Giera M, Semrau S, Tertoolen LGJ, Orlova VV, Bellin M, Mummery CL
Journal:Cell Stem Cell
PubMed ID:32459996
'Cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) are functionally immature, but this is improved by incorporation into engineered tissues or forced contraction. Here, we showed that tri-cellular combinations of hiPSC-derived CMs, cardiac fibroblasts (CFs), and cardiac endothelial cells also enhance maturation in easily constructed, scaffold-free, three-dimensional microtissues (MTs). ... More