PSC Cardiomyocyte Differentiation Kit

Citations & References (10)

Gibco™

PSC Cardiomyocyte Differentiation Kit

製品番号（カタログ番号）	数量
A2921201	1 kit

製品番号（カタログ番号） A2921201

価格（JPY）

69,800

お問い合わせください ›

1 kit

Gibco™ PSC心筋細胞分化キットは、血清も異物もない培地セットで構成されており、ヒト多能性幹細胞（PSC）をわずか8日で心筋細胞に効率的に分化させることができます。複数の成分と長時間のアッセイを必要とする他の方法とは異なり、PSC心筋細胞分化キットは、すぐに使用可能な培地フォーマットで多能性幹細胞から心筋細胞を短時間で生成できます。

融解や混合を必要としない3種類の1倍培地で構成されており、各培地は合計14日間連続して使用されます。その結果、関連する生理的マーカーを発現する機能性心筋細胞が培養下で収縮し、培養下で15日間以上維持することができます（維持培地は別売りです（カタログ番号A2920801））。

融解や混合が不要
PSC心筋細胞分化キットは凍結保存を必要としない1倍培地で構成されているため、試薬の解凍や混合が不要です。培地を温めてPSC培養物に加えるだけです。

心筋細胞の迅速な生成
PSC心筋細胞分化キットはわずか8日で心筋細胞の収縮を生成できます。分化した心筋細胞は培養下で15日間以上維持できます。

高品質の心筋細胞の生成
PSC心筋細胞分化キットを使用して生成された心筋細胞は、機能的に関連があり、TNNT2、Nkx2.5、MYH6、α-アクチニンなどの主要マーカーを発現し、培養下で収縮を示します。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ心筋細胞

数量1 kit

出荷条件室温

製品タイプPSC Cardiomyocyte Differentiation Kit

Unit SizeEach

組成および保存条件

• 500 mL心筋細胞維持培地
• 100 mL心筋細胞分化培地A
• 100 mL心筋細胞分化培地B

遮光し、2～8℃で保存。

よくあるご質問（FAQ）

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?

We recommend always using H9 or H7 ESC line as a control in your experiments. We recommend adjusting the cell density or extending the induction time for difficult-to-differentiate iPSC lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I maintain differentiated cells in culture using the Gibco PSC Cardiomyocyte Differentiation Kit?

Differentiated cells can be maintained for a month or longer for long-term studies. We recommend the use of Gibco Geltrex Matrix for long-term cultures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which population of cardiomyocytes is produced using the PSC Cardiomyocyte Differentiation Kit?

The population of cardiomyocytes produced is a mix of atrial and ventricular cells. Over time, cultures become more ventricular.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all PSC Cardiomyocyte Differentiation Kit FAQs

引用および参考文献 (10)

引用および参考文献

Abstract

Cardiac disease modeling using induced pluripotent stem cell-derived human cardiomyocytes.

Authors:Dell'Era P, Benzoni P, Crescini E, Valle M, Xia E, Consiglio A, Memo M,

Journal:

PubMed ID:25815118

Causative mutations and variants associated with cardiac diseases have been found in genes encoding cardiac ion channels, accessory proteins, cytoskeletal components, junctional proteins, and signaling molecules. In most cases the functional evaluation of the genetic alteration has been carried out by expressing the mutated proteins in in-vitro heterologous systems. While ... More

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi004-A from a carrier of the KCNQ1-R594Q mutation.

Authors:Mura M, Lee YK, Pisano F, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Tse HF, Gnecchi M

Journal:Stem Cell Res

PubMed ID:30974404

'We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a male carrier of the heterozygous mutation c.1781?G?>?A p.R594Q on the KCNQ1 gene. hiPSCs, generated using four retroviruses each encoding for OCT4, SOX2, KLF4 and cMYC, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating ... More

Reprogramming of Urine-Derived Renal Epithelial Cells into iPSCs Using srRNA and Consecutive Differentiation into Beating Cardiomyocytes.

Authors:Steinle H, Weber M, Behring A, Mau-Holzmann U, von Ohle C, Popov AF, Schlensak C, Wendel HP, Avci-Adali M

Journal:Mol Ther Nucleic Acids

PubMed ID:31476669

'The generation of induced pluripotent stem cells (iPSCs) from patient''s somatic cells and the subsequent differentiation into desired cell types opens up numerous possibilities in regenerative medicine and tissue engineering. Adult cardiomyocytes have limited self-renewal capacity; thus, the efficient, safe, and clinically applicable generation of autologous cardiomyocytes is of great ... More

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi007-A from a Long QT Syndrome type 1 patient carrier of two common variants in the NOS1AP gene.

Authors:Mura M, Pisano F, Stefanello M, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Brink PA, Gnecchi M

Journal:Stem Cell Res

PubMed ID:30878014

We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic Long QT Syndrome (LQTS) type 1 patient, belonging to a South African (SA) founder population segregating the heterozygous mutation c.1022C?>?T p.A341V on the KCNQ1 gene. The patient is also homozygous for the two minor variants rs4657139 and rs16847548 on ... More

Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells.

Authors:Wei H, Wang C, Guo R, Takahashi K, Naruse K

Journal:Biochem Biophys Res Commun

PubMed ID:31623826

Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart ... More

View all PSC Cardiomyocyte Differentiation Kit citations