Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer
Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer
Citations & References (35)
Invitrogen™

Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer

テトラフルオロフェニル（TFP）エステルは、生体分子の一級アミンにフルオロフォアやハプテンを付加するために一般的に使用されるスクシンイミジルエステル（SEまたはNHSエステル）試薬に比べて改良されています。どちらの反応化学も、色素またはハプテンと対象化合物との間に強いアミド結合を同じように形成しますが、TFPエステルは結合反応中の自然発生的加水分解の影響を受けにくくなっています。Alexa詳細を見る
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製品番号（カタログ番号）数量
A300051 mg
製品番号（カタログ番号） A30005
価格（JPY）
84,800
Each
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数量:
1 mg
テトラフルオロフェニル（TFP）エステルは、生体分子の一級アミンにフルオロフォアやハプテンを付加するために一般的に使用されるスクシンイミジルエステル（SEまたはNHSエステル）試薬に比べて改良されています。どちらの反応化学も、色素またはハプテンと対象化合物との間に強いアミド結合を同じように形成しますが、TFPエステルは結合反応中の自然発生的加水分解の影響を受けにくくなっています。Alexa Fluor TFPエステルは、一般的には、反応に使用される塩基性pHで、スコチニミジルエステルをはるかに上回り、数時間にわたって安定しています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学反応性アミン
標識または色素Alexa Fluor™ 488
製品タイプカルボン酸テトラフルオロフェニルエステル
数量1 mg
反応性部分活性エステル、TFPエステル
出荷条件室温
標識タイプAlexa Fluor色素
製品ラインAlexa Fluor
Unit SizeEach
組成および保存条件
フリーザー(-5∼-30度)に保存し、遮光してください。

よくあるご質問（FAQ）

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (35)

引用および参考文献
Abstract
Identification, cloning, and characterization of two N-acetylgalactosamine-binding lectins from the albumen gland of Helix pomatia.
Authors:Markiv A, Peiris D, Curley GP, Odell M, Dwek MV,
Journal:J Biol Chem
PubMed ID:21372134
Helix pomatia agglutinin (HPA), the lectin from the albumen gland of the Roman snail, has been used in histochemical studies relating glycosylation changes to the metastatic potential of solid tumors. To facilitate the use of HPA in a clinical (diagnostic) setting, detailed analysis of the lectin, including cloning and recombinant ... More
Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs.
Authors:Kosturko LD, Maggipinto MJ, Korza G, Lee JW, Carson JH, Barbarese E
Journal:Mol Biol Cell
PubMed ID:16775011
'Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a trans-acting RNA-binding protein that mediates trafficking of RNAs containing the cis-acting A2 response element (A2RE). Previous work has shown that A2RE RNAs are transported to myelin in oligodendrocytes and to dendrites in neurons. hnRNP E1 is an RNA-binding protein that regulates translation of ... More
Photolithographic patterning of polyethylene glycol hydrogels.
Authors:Hahn MS, Taite LJ, Moon JJ, Rowland MC, Ruffino KA, West JL
Journal:Biomaterials
PubMed ID:16375965
'A simple, inexpensive photolithographic method for surface patterning deformable, solvated substrates is demonstrated using photoactive poly(ethylene glycol) (PEG)-diacrylate hydrogels as model substrates. Photolithographic masks were prepared by printing the desired patterns onto transparencies using a laser jet printer. Precursor solutions containing monoacryloyl-PEG-peptide and photoinitiator were layered onto hydrogel surfaces. The ... More
Physical interactions of the peroxisomal targeting signal 1 receptor pex5p, studied by fluorescence correlation spectroscopy.
Authors:Wang D, Visser NV, Veenhuis M, van der Klei IJ
Journal:J Biol Chem
PubMed ID:12930827
'We have studied Hansenula polymorpha Pex5p and Pex8p using fluorescence correlation spectroscopy (FCS). Pex5p is the Peroxisomal Targeting Signal 1 (PTS1) receptor and Pex8p is an intraperoxisomal protein. Both proteins are essential for PTS1 protein import and have been shown to physically interact. We used FCS to analyze the molecular ... More
Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging.
Authors:Smith BA, Daugherty-Clarke K, Goode BL, Gelles J,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23292935
'Actin filament nucleation by actin-related protein (Arp) 2/3 complex is a critical process in cell motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process. Triggered by the verprolin homology, central, and acidic (VCA) region ... More
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