PSC Definitive Endoderm Induction Kit

Gibco™ PSC Definitive Endoderm Induction Kitは、2種類のゼノフリー培地で構成されており、ヒト多能性幹細胞（PSC）を胚体内胚葉に効率的に誘導できます詳細を見る

製品番号（カタログ番号）	数量
A3062601	1 kit

製品番号（カタログ番号） A3062601

価格（JPY）

124,100

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1 kit

Gibco™ PSC Definitive Endoderm Induction Kitは、2種類のゼノフリー培地で構成されており、ヒト多能性幹細胞（PSC）を胚体内胚葉に効率的に誘導できます。PSC胚体内胚葉誘導キットは、複数の成分が必要で5日以上かかる他の方法とは異なり、わずか2つの成分とわずか2日の期間で90%以上のCXCR4+/PDGFRα-胚体内胚葉を生成できます。各培地は1倍完全培地として提供され、他の成分の混合は不要です。結果として得られる胚体内胚葉は、関連する生理的マーカーを発現する中腸／後腸、膵臓内胚葉、または肝芽前駆体に分化できます。

高品質胚体内胚葉の生成
PSC Definitive Endoderm Induction Kitを使用して生成した胚体内胚葉は、中腸／後腸（CDX2を発現）、膵臓内胚葉（PDX2を発現）、肝芽前駆体（AFPを発現）などのダウンストリーム系統に分化できます。

胚体内胚葉の迅速な生成
PSC Definitive Endoderm Induction Kitは、わずか2日で胚体内胚葉を生成できます。

高純度の胚体内胚葉を生成
PSC Definitive Endoderm Induction Kitは多能性幹細胞から高純度の胚体内胚葉集団を生成でき、複数の株全体で90%以上のCXCR4+/PDGFRαを示し、キーマーカーのSOX17およびFOXA2を高発現します。

必要な成分の混合は不要
各培地は1倍濃度で提供され、試薬の混合は不要です。培地を融解してPSC培養に加えるだけです。

細胞タイプ幹細胞（ヒト）

数量1 kit

出荷条件ドライアイス

製品タイプPSC Definitive Endoderm Induction Kit

Unit SizeEach

組成および保存条件

• PSC胚体内胚葉誘導培地A
• PSC胚体内胚葉誘導培地B

よくあるご質問（FAQ）

Can I cryopreserve definitive endoderm cells?

We don't recommend cryopreserving cells at the definitive endoderm stage. After definitive endoderm induction is complete, we recommend using the cells either for characterization or immediately starting downstream differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can these cells be maintained in definitive endoderm medium?

No. Once definitive endoderm cells are induced, we recommend using the cells either for characterization or immediately starting downstream differentiation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I characterize definitive endoderm cells?

Definitive endoderm cells generated using the PSC Definitive Endoderm Induction kit can be characterized by FACS or immunocytochemistry by looking at high expression of endoderm markers, including CXCR4, SOX17, and FOXA2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see floating cells after addition of medium B when using the PSC Definitive Endoderm Induction Kit. Is that normal?

It is normal to see floating cells after addition of medium B. Floating cells are not detrimental to definitive endoderm efficiency. Higher confluency (>15-30%) at the time of induction will generally lead to higher number of floating cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to incubate cells for exactly 24 hours in medium A or medium B when using the PSC Definitive Endoderm Induction Kit?

If necessary, cells can be incubated for 24 +/-3h.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all PSC Definitive Endoderm Induction Kit FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.

Authors:Martins GLS, Paredes BD, Sampaio GLA, Nonaka CKV, da Silva KN, Allahdadi KJ, França LSA, Soares MBP, Dos Santos RR, Souza BSF

Journal:Stem Cell Res

PubMed ID:31706097

Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro. ... More

Ultrasensitive and rapid quantification of rare tumorigenic stem cells in hPSC-derived cardiomyocyte populations.

Authors:Wang Z, Gagliardi M, Mohamadi RM, Ahmed SU, Labib M, Zhang L, Popescu S, Zhou Y, Sargent EH, Keller GM, Kelley SO

Journal:Sci Adv

PubMed ID:32440533

The ability to detect rare human pluripotent stem cells (hPSCs) in differentiated populations is critical for safeguarding the clinical translation of cell therapy, as these undifferentiated cells have the capacity to form teratomas in vivo. The detection of hPSCs must be performed using an approach compatible with traceable manufacturing of ... More