IonCode™ Barcode Adapters 1-384 Kit (for Agrigenomics)

The IonCode Barcode Adapters 1-384 Kit for Agrigenomics provides a set of unique barcode adapters designed for optimal performance with Ion semiconductor sequencers. When used in combination with Ion Barcode Adapters 385-768 and the AgriSeq HTS Library Kit, these barcodes enable users to pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, helping increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance helps ensure identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification

Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 768 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode has been individually evaluated to help ensure no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to enable balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figure below).